Genome-guided bioprospecting for industrially important enzymes from a thermophilic Bacillus subtilis strain SR-7, an isolate from hot spring of Madhya Pradesh, India

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-09-11 DOI:10.1016/j.genrep.2024.102029

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Abstract

The study focused on isolating thermophilic bacteria from the hot springs of Madhya Pradesh, India, with the aim of identifying strains capable of producing industrially valuable enzymes. Among the 15 isolated strains, SR-7 identified as Bacillus subtilis exhibited significant enzyme activity, leading to its selection for further investigation. Genomic analysis revealed key characteristics of SR-7, including a 3,593,163 base pair chromosome with a GC content of 44.14 %. The genome was found to contain 55 tRNA genes, four rRNA clusters, and 3744 protein-coding genes. These genes encompass essential metabolic pathways and genes responsible for thermophilic adaptation, highlighting the potential for industrial applications. Further experimentation demonstrated SR-7 have the ability to produce various enzymes of industrial significance, such as amylase, lipase, cellulase, protease, lecithinase, gelatinase, and pectinase. Importantly, enzyme production was observed across a broad pH range (6.5 to 10) and temperature spectrum, indicating the versatility and adaptability of SR-7 to different environmental conditions. The findings suggest that SR-7 holds promise as a valuable resource for the pharmaceutical and industrial sectors. Its capacity to produce thermostable enzymes suitable for a range of applications underscores its potential contribution to biotechnological processes. By harnessing the enzymatic capabilities of thermophilic bacteria like SR-7, industries can pursue more sustainable and efficient production methods, reducing reliance on traditional chemical processes and enhancing overall productivity. Further research into the enzymatic properties and genetic mechanisms of SR-7 may unveil additional avenues for its utilization in various industrial processes.

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以基因组为指导，从印度中央邦温泉分离出的嗜热枯草芽孢杆菌 SR-7 菌株中寻找具有重要工业价值的酶

这项研究的重点是从印度中央邦的温泉中分离出嗜热细菌，目的是找出能够生产具有工业价值的酶的菌株。在分离出的 15 株菌株中，SR-7 被鉴定为枯草芽孢杆菌，具有显著的酶活性，因此被选中进行进一步研究。基因组分析揭示了 SR-7 的主要特征，包括一个 3,593,163 碱基对的染色体，其 GC 含量为 44.14%。基因组包含 55 个 tRNA 基因、4 个 rRNA 簇和 3744 个编码蛋白质的基因。这些基因包括重要的新陈代谢途径和负责嗜热适应的基因，凸显了工业应用的潜力。进一步的实验证明，SR-7 有能力生产各种具有工业意义的酶，如淀粉酶、脂肪酶、纤维素酶、蛋白酶、卵磷脂酶、明胶酶和果胶酶。重要的是，在广泛的 pH 值范围（6.5 至 10）和温度范围内都能观察到酶的产生，这表明 SR-7 具有多功能性，能适应不同的环境条件。研究结果表明，SR-7 有望成为制药和工业部门的宝贵资源。它生产适用于一系列应用的恒温酶的能力突出了其对生物技术过程的潜在贡献。通过利用 SR-7 等嗜热细菌的酶能力，工业界可以采用更可持续、更高效的生产方法，减少对传统化学工艺的依赖，提高整体生产率。对 SR-7 的酶特性和遗传机制的进一步研究可能会为其在各种工业流程中的应用开辟新的途径。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.