Effect of dietary lycopene supplementation on growth, antioxidant and immunity of juvenile hybrid grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) fed with high lipid diets

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-09-17 DOI:10.1016/j.aqrep.2024.102367

Menglong Zhou , Hao Liu , Baiquan Lu , Biao Li , Weibin Huang , Hengyang Song , Wenshan Cai , Beiping Tan , Yuanzhi Yang , Xiaohui Dong

{"title":"Effect of dietary lycopene supplementation on growth, antioxidant and immunity of juvenile hybrid grouper (♀Epinephelus fuscoguttatus ×♂E. lanceolatus) fed with high lipid diets","authors":"Menglong Zhou , Hao Liu , Baiquan Lu , Biao Li , Weibin Huang , Hengyang Song , Wenshan Cai , Beiping Tan , Yuanzhi Yang , Xiaohui Dong","doi":"10.1016/j.aqrep.2024.102367","DOIUrl":null,"url":null,"abstract":"<div>The aim of this experiment was to investigate the effects of dietary lycopene (LCP) on growth performance, antioxidant, and immunity of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) fed with high-lipid diets (HLD, CL16 %). Hybrid groupers (20.56±0.17 g) were selected and fed four diets containing 0, 100, 200 and 400 mg/kg of LCP, named L0, L1, L2 and L3. The results showed that: FBW and SGR were significantly higher in all dietary LCP group compared to the L0 group (P< 0.05). In serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) content were significantly higher in group L2 than in group L0, and alkaline phosphatase (AKP) content in L3 group was significantly lower (P< 0.05). In the liver, malondialdehyde (MDA) content were significantly lower in all LCP groups than in the L0 group (P< 0.05); catalase (CAT), AKP, and acid phosphatase (ACP) activities were significantly higher in all LCP group than in the L0 group (P< 0.05); SOD, Aspartate aminotransferase (GOT) and glutamate-pyruvate transaminase (GPT) activities in the liver were significantly higher in the L2 group than in the L0 group (P< 0.05). Dietary supplementation of 200–400 mg/kg LCP significantly up-regulated mRNA levels of hepatic sod, gsh-px, cat, nuclear factor erythroid 2-related factor 2 (nrf2), heat stimulating protein 90 (hsp90) and transforming growth factor-β (tgfβ), and significantly down-regulated mRNA levels of hepatic kelch-like ECH-associated protein 1 (keap1) and interleukin-1β (il1β) (P< 0.05). The optimal levels of LCP were 282.22 mg/kg, 246.83 mg/kg and 248.5 mg/kg for FBW, serum GSH-Px activity and hepatic immunoglobulin M (IgM) content, respectively. In conclusion, the addition of 200–300 mg/kg LCP could promote the growth performance of hybrid grouper, improve the antioxidant capacity, and alleviate the oxidative damage of liver caused by HLD.</div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102367"},"PeriodicalIF":3.2000,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352513424004551/pdfft?md5=905c540d28a33f984b286807aee229e7&pid=1-s2.0-S2352513424004551-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352513424004551","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

The aim of this experiment was to investigate the effects of dietary lycopene (LCP) on growth performance, antioxidant, and immunity of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) fed with high-lipid diets (HLD, CL16 %). Hybrid groupers (20.56±0.17 g) were selected and fed four diets containing 0, 100, 200 and 400 mg/kg of LCP, named L0, L1, L2 and L3. The results showed that: FBW and SGR were significantly higher in all dietary LCP group compared to the L0 group (P< 0.05). In serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) content were significantly higher in group L2 than in group L0, and alkaline phosphatase (AKP) content in L3 group was significantly lower (P< 0.05). In the liver, malondialdehyde (MDA) content were significantly lower in all LCP groups than in the L0 group (P< 0.05); catalase (CAT), AKP, and acid phosphatase (ACP) activities were significantly higher in all LCP group than in the L0 group (P< 0.05); SOD, Aspartate aminotransferase (GOT) and glutamate-pyruvate transaminase (GPT) activities in the liver were significantly higher in the L2 group than in the L0 group (P< 0.05). Dietary supplementation of 200–400 mg/kg LCP significantly up-regulated mRNA levels of hepatic sod, gsh-px, cat, nuclear factor erythroid 2-related factor 2 (nrf2), heat stimulating protein 90 (hsp90) and transforming growth factor-β (tgfβ), and significantly down-regulated mRNA levels of hepatic kelch-like ECH-associated protein 1 (keap1) and interleukin-1β (il1β) (P< 0.05). The optimal levels of LCP were 282.22 mg/kg, 246.83 mg/kg and 248.5 mg/kg for FBW, serum GSH-Px activity and hepatic immunoglobulin M (IgM) content, respectively. In conclusion, the addition of 200–300 mg/kg LCP could promote the growth performance of hybrid grouper, improve the antioxidant capacity, and alleviate the oxidative damage of liver caused by HLD.

查看原文本刊更多论文

补充番茄红素对高脂日粮饲喂杂交石斑鱼（♀Epinephelus fuscoguttatus ×♂E. lanceolatus）幼鱼生长、抗氧化和免疫力的影响

本实验旨在研究日粮番茄红素（LCP）对杂交石斑鱼（Epinephelus fuscoguttatus♀ × E. lanceolatus♂）生长性能、抗氧化性和免疫力的影响。选取杂交石斑鱼（20.56±0.17 g），分别投喂含 0、100、200 和 400 mg/kg LCP 的四种日粮，命名为 L0、L1、L2 和 L3。结果表明与 L0 组相比，所有日粮 LCP 组的 FBW 和 SGR 均显著提高（P< 0.05）。在血清中，L2 组的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和总抗氧化能力（T-AOC）含量明显高于 L0 组，而 L3 组的碱性磷酸酶（AKP）含量明显低于 L0 组（P< 0.05）。在肝脏中，所有 LCP 组的丙二醛（MDA）含量均明显低于 L0 组（P< 0.05）；所有 LCP 组的过氧化氢酶（CAT）、碱性磷酸酶（AKP）和酸性磷酸酶（ACP）活性均明显高于 L0 组（P< 0.05）；肝脏中的 SOD、天门冬氨酸氨基转移酶（GOT）和谷氨酸丙酮酸转氨酶（GPT）活性在 L2 组明显高于 L0 组（P< 0.05）。膳食补充 200-400 mg/kg LCP 可显著上调肝脏 sod、gsh-px、cat、核因子红细胞 2 相关因子 2（nrf2）、热刺激蛋白 90（hsp90）和转化生长因子-β（tgfβ）的 mRNA 水平，显著下调肝脏 kelch-like ECH-associated protein 1（keap1）和白细胞介素-1β（il1β）的 mRNA 水平（P< 0.05）。对于 FBW、血清 GSH-Px 活性和肝脏免疫球蛋白 M (IgM) 含量，LCP 的最佳水平分别为 282.22 mg/kg、246.83 mg/kg 和 248.5 mg/kg。总之，添加 200-300 mg/kg LCP 可促进杂交石斑鱼的生长性能，提高抗氧化能力，减轻 HLD 对肝脏的氧化损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.