Salicylic acid functionalized chitosan nanocomposite increases bioactive components and insect resistance of Agastache rugosa

Abstract

The abundance of monoterpenoids and phenolic compounds determines the medicinal quality and anti-insect properties of Agastache rugosa, which can be compromised by biotic stress such as herbivore attacks. The traditional use of chemical pesticides to mitigate herbivore interference is increasingly incompatible with sustainable agriculture. In response, nanotechnology-based biostimulants, which can activate metabolic processes to enhance plant growth and stress resistance, offer a more cost-effective and environmentally-friendly alternative. However, to date, it remains unknown how nano-biostimulants improve the therapeutic value and insect resistance of medicinal plants simultaneously. This study investigates the effect of 0–1000 mg/L of a nano-biostimulant salicylic acid functionalized chitosan nanocomposite (SCN) on the pharmacological and anti-herbivore properties of medicinal plant A. rugosa. Results showed that 100 mg/L SCN significantly inhibited Spodoptera litura growth by 62.9 %, and increased plant shoot and root biomass by 107.2 % and 77.6 %, respectively. Moreover, 100 mg/L SCN significantly upregulated the expression of the key genes (e.g., LS, L3OH, and CHS) involved in monoterpene and phenolic compounds biosynthesis by 1.4–10.1 folds, thus boosting the production of active compounds such as pulegone, β-myrcene, and chlorogenic acid by 1.5–24.4 folds. These enhancements were superior to salicylic acid or chitosan alone. Altogether, our findings promote the sustainable and eco-friendly application of nano-biostimulant in improving the quality of medicinal plants and green pest control in agroecosystems.