Evaluation of cobas® Liat® Cdiff, STANDARD™ M10 C. difficile and Xpert® C. difficile BT assays for rapid detection of toxigenic Clostridioides difficile

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2024-09-12 DOI:10.1016/j.mimet.2024.107043

Juulia Suominen , Suvi Korhonen , Heini Kutvonen , Hanna Jarva , Anu Pätäri-Sampo , Raisa Loginov

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引用次数: 0

Abstract

We evaluated the analytical performance of three commercial molecular assays for rapid detection of Clostridioides difficile toxin B in stool samples. The results were compared with results from the BD MAX™ Cdiff assay. We analyzed forty negative and thirty-two positive stool samples with three rapid assays: Roche cobas® Liat® Cdiff, SD Biosensor STANDARD™ M10 C. difficile and Cepheid Xpert® C. difficile BT. The assays demonstrated a sensitivity of 96.9 %, 96.9 % and 100.0 % and a specificity of 100 %, 97.5 % and 97.5 %, respectively. There is limited data available on the analytical performance of the newly introduced STANDARD™ M10 C. difficile assay. In this study, all three rapid assays demonstrated similarly high analytical performance and can be used for detection of toxigenic C. difficile.

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评估用于快速检测毒性艰难梭菌的 cobas® Liat® Cdiff、STANDARD™ M10 艰难梭菌和 Xpert® 艰难梭菌 BT 检测方法

我们对快速检测粪便样本中艰难梭菌毒素 B 的三种商用分子测定的分析性能进行了评估。结果与 BD MAX™ Cdiff 检测法的结果进行了比较。我们使用三种快速检测方法分析了 40 份阴性和 32 份阳性粪便样本：罗氏 cobas® Liat® Cdiff、SD Biosensor STANDARD™ M10 艰难梭菌和 Cepheid Xpert® 艰难梭菌 BT。这些检测方法的灵敏度分别为 96.9 %、96.9 % 和 100.0 %，特异性分别为 100 %、97.5 % 和 97.5 %。关于新推出的 STANDARD™ M10 艰难梭菌检测法的分析性能，目前可用的数据还很有限。在这项研究中，所有三种快速检测方法都表现出了类似的高分析性能，可用于检测致毒艰难梭菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.