Ecdysone promotes gene- and pathogen-specific immune responses to Micrococcus luteus and Bacillus subtilis in Drosophila S2 cells

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
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Abstract

In Drosophila, the 20-hydroxyecdysone (20E) hormone regulates numerous essential biological processes. Here, we studied the contribution of 20E to the activity of immune signaling pathways and antimicrobial activity using the model Drosophila S2 cells. We found that while 20E alone has no essential effect on this system, pretreating S2 cells with 20E followed by incubation with Escherichia coli or Micrococcus luteus stimulates the induction of a limited number of antimicrobial peptide (AMP) genes, such as Diptericin (Dpt) and Drosomycin (Drs). Contrary to this, cells pretreatment with 20E simulates the activity of numerous Bacillus subtilis-induced AMP genes. Interestingly, it also significantly promotes the expression of components of both the Toll (Dif, Dorsal, etc.) and the IMD pathways (Relish, IMD, etc.) in the presence of Bacillus subtilis. Unexpectedly, simultaneous treatment of S2 cells by 20E and all three bacteria shows another pattern of activity and leads to a suppression of Drosocin (Dro) induction, in particular. Our study reveals that the contribution of 20E to immune genes activity varies for different genes and depends on the mode of 20E interplay with the pathogen and the nature of the pathogen itself.

Abstract Image

蜕皮激素促进果蝇 S2 细胞对黄体微球菌和枯草杆菌的基因和病原体特异性免疫反应
在果蝇体内,20-羟基蜕皮激素(20E)调节着许多重要的生物过程。在这里,我们利用果蝇模型 S2 细胞研究了 20E 对免疫信号通路活性和抗菌活性的贡献。我们发现,虽然 20E 本身对该系统没有本质影响,但用 20E 预处理 S2 细胞,然后用大肠杆菌或黄体小球菌孵育,会刺激诱导有限数量的抗菌肽(AMP)基因,如 Diptericin (Dpt) 和 Drosomycin (Drs)。与此相反,用 20E 对细胞进行预处理可模拟大量枯草杆菌诱导的 AMP 基因的活性。有趣的是,在枯草芽孢杆菌存在的情况下,20E 还能显著促进 Toll(Dif、Dorsal 等)和 IMD(Relish、IMD 等)通路成分的表达。意想不到的是,同时用 20E 和这三种细菌处理 S2 细胞会显示出另一种活动模式,尤其会导致抑制 Drosocin(Dro)的诱导。我们的研究表明,20E 对不同基因的免疫基因活性的贡献各不相同,取决于 20E 与病原体相互作用的模式以及病原体本身的性质。
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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