Metabolite profiling and bioactivity assessment of cyanobacteria from the Azores reveals unique producers of cytotoxic and lipid reducing compounds

IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Rúben Luz , Vítor Gonçalves , Vitor Vasconcelos , Ralph Urbatzka
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Abstract

Cyanobacteria are a group of photosynthetic bacteria and a rich secondary metabolites source. The Bank of Algae and Cyanobacteria of the Azores (BACA) culture collection holds a significant number of strains, including many novel genera and species. 56 strains from freshwater, brackish, and thermal habitats were selected, and grown under standard conditions. Biomass was extracted with methanol, and cytotoxicity was assessed on two carcinoma cell lines, HepG2 and HCT116. The reduction of lipids was tested in zebrafish larvae, and in a steatosis model with fatty acid overloaded human liver cells. The cyanobacterial metabolome was analyzed by HR-ESI-LC-MS/MS and compared using CompareMS2. High similarities were observed in strains of the same genus when isolated from similar habitats, clustering in concordance to the taxonomical order, while no relation could be observed between strains from different genera originated from the same habitat. The extracts of Cyanobium sp. BACA0019, Pseudocalidococcus azoricus BACA0433 and Pegethrix atlantica BACA0077 reduced neutral lipids >40 % in zebrafish at 25 μg/mL, while from Symphyonema sp. BACA0090 and Aliinostoc sp. BACA0355 induced mortality. Lipid reduction in the steatosis model was observed in many strains, with significant results varying from 50 % to 100 %. Several strains reduced cell viability with the strongest effects from Scytonematopsis sp. BACA0005 (HepG2, 59.8 % and HCT116, 68.1 %), Aliinostoc sp. BACA0035 (HepG2, 43.3 %, and HCT116, 59.4 %) and Aliinostoc sp. BACA0355 (HepG2, 46.2 %, and HCT116, 75.5 %). The feature-based molecular networking identified several cluster of mass peaks related to the observed bioactivities. Chlorophyll derivatives and glycerolipids from Cyanobium sp. BACA0019, Pseudocalidococcus azoricus BACA0433 and Pegethrix atlantica BACA0077 were correlated with the reduction of lipids in zebrafish larvae, while several oligopeptides and fatty amides of Symphyonema sp. BACA0090 and Aliinostoc sp. BACA0355 with toxicity. Many clusters associated to the bioactivities remained unidentified, which may represent novel compounds, highlighting the chemodiversity of the BACA culture collection.

Abstract Image

亚速尔群岛蓝藻的代谢物谱分析和生物活性评估揭示了独特的细胞毒性和脂质还原化合物生产者
蓝藻是一组光合细菌,也是丰富的次级代谢物来源。亚速尔群岛藻类和蓝藻菌库(BACA)的培养物中有大量菌株,包括许多新属和新种。研究人员从淡水、咸水和热环境中筛选出 56 株菌株,并在标准条件下进行培养。用甲醇提取生物质,并对两种癌细胞系(HepG2 和 HCT116)进行细胞毒性评估。在斑马鱼幼体和脂肪酸超载的人类肝细胞脂肪变性模型中测试了蓝藻对脂质的减少作用。蓝藻代谢组通过 HR-ESI-LC-MS/MS 进行分析,并使用 CompareMS2 进行比较。从相似的生境中分离出的同属菌株具有很高的相似性,按照分类学顺序聚类,而从同一生境中分离出的不同属的菌株之间则没有任何关系。BACA0019、BACA0433 和 Pegethrix atlantica BACA0077 的提取物能在 25 μg/mL 的浓度下降低斑马鱼 40% 的中性脂质,而 BACA0090 和 BACA0355 的提取物则会导致斑马鱼死亡。在脂肪变性模型中,许多菌株都观察到脂质减少,显著结果从 50 % 到 100 % 不等。一些菌株降低了细胞活力,其中影响最大的是 Scytonematopsis sp. BACA0005(HepG2,59.8%;HCT116,68.1%)、Aliinostoc sp.基于特征的分子网络确定了与所观察到的生物活性相关的几个质量峰群。叶绿素衍生物和甘油脂与斑马鱼幼虫体内脂质的减少有关,而 Symphyonema sp.BACA0090、Pseudocalidococcus azoricus BACA0433 和 Pegethrix atlantica BACA0077 的几种寡肽和脂肪酰胺与毒性有关。许多与生物活性相关的簇仍未确定,这可能代表了新型化合物,凸显了 BACA 培养物收集的化学多样性。
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来源期刊
Algal Research-Biomass Biofuels and Bioproducts
Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
9.40
自引率
7.80%
发文量
332
期刊介绍: Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment
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