In vivo imaging of vagal-induced myenteric plexus responses in gastrointestinal tract with an optical window

IF 14.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2024-09-16 DOI:10.1038/s41467-024-52397-0

Longjie Jiang, Jie Yang, Xiujuan Gao, Jiangfeng Huang, Qian Liu, Ling Fu

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Abstract

The vagus nerve (VN) extensively innervates the gastric enteric nervous system (ENS), but its influence on gastric ENS functionality and motility in vivo remains unclear due to technical challenges. Here we describe a method for stable, long-term observation of gastric ENS activity and muscle dynamics at cellular resolution, which can also be extended to intestinal applications. This method involves ENS-specific labeling and the implantation of an abdominal wall window for optical recording in male mice. In vivo calcium imaging reveals a linear relationship between vagal stimulation frequency and myenteric neuron activation in gastric antrum. Furthermore, the motility of gastric antrum is significantly enhanced and shows a positive correlation with the intensity and number of activated myenteric neurons. While vagal stimulation also activates proximal colonic myenteric neurons, this activation is not frequency-dependent and does not induce proximal colonic motility. The method and results provide important insights into VN-ENS interactions in vivo, advancing our understanding of gastrointestinal motility regulation.

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利用光学窗口对迷走神经诱发的胃肠道肠肌丛反应进行活体成像

迷走神经（VN）广泛支配胃肠神经系统（ENS），但由于技术上的挑战，迷走神经对胃肠神经系统功能和体内运动的影响仍不清楚。在这里，我们描述了一种以细胞分辨率长期稳定观察胃 ENS 活动和肌肉动态的方法，这种方法也可扩展到肠道应用。这种方法包括对 ENS 进行特异性标记，并在雄性小鼠腹壁上植入一个光学记录窗口。体内钙成像显示，迷走神经刺激频率与胃窦肌神经元激活之间存在线性关系。此外，胃窦的蠕动明显增强，并与激活的肠肌神经元的强度和数量呈正相关。虽然迷走神经刺激也会激活结肠近端肠肌神经元，但这种激活并不依赖于频率，也不会诱导结肠近端运动。该方法和结果提供了体内 VN-ENS 相互作用的重要见解，加深了我们对胃肠运动调节的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.