Portable real-time determination of Escherichia coli O157:H7 and Staphylococcus aureus based on smartphones and hydrogels

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-09-13 DOI:10.1016/j.saa.2024.125119

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Abstract

The aptamers functionalized orange-emission carbon dots (OCDs) and green-emission carbon dots (GCDs) had dual-emission peaks with single excitation. Tungsten disulfide nanosheets (WS₂ NSs)-triggered fluorescence quenching achieved the ratiometric fluorescence determination of Escherichia coli O157:H7 (E. coli O157:H7) and Staphylococcus aureus (S. aureus) with wide ranges of 18–1.8 × 10⁶ and 37–3.7 × 10⁷ CFU/mL and low detection limits of 8 and 20 CFU/mL, respectively. The results in real sample with recoveries of 90–101 % and RSD < 4.12 % were no significant difference from standard plate counting method. Meanwhile, the dual-color CDs were further adopted in the smartphone-assisted hydrogel platform and achieved speedy, sensitive, portable and real-time determination of E. coli O157:H7 and S. aureus in real samples. This work has not only developed ratiometric fluorescence detection and constructed a portable hydrogel platform, but also provided a unique strategy in developing a time-efficient and easy-to-use portable device in food safety monitoring.

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基于智能手机和水凝胶的便携式大肠杆菌 O157:H7 和金黄色葡萄球菌实时检测系统

功能化了橙色发射碳点（OCDs）和绿色发射碳点（GCDs）的配合物在单激发下具有双发射峰。二硫化钨纳米片（WS2 NSs）触发的荧光淬灭实现了大肠杆菌 O157:H7 和金黄色葡萄球菌（S. aureus）的比率荧光检测，检测范围分别为 18-1.8 × 106 和 37-3.7 × 107 CFU/mL，检测限分别为 8 和 20 CFU/mL。实际样品的回收率为 90-101 %，RSD < 4.12 %，与标准平板计数法无显著差异。同时，在智能手机辅助水凝胶平台中进一步采用了双色光盘，实现了对实际样品中大肠杆菌 O157:H7 和金黄色葡萄球菌的快速、灵敏、便携和实时测定。这项工作不仅开发了比率荧光检测技术，构建了便携式水凝胶平台，还为开发省时、易用的食品安全监控便携设备提供了独特的策略。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.

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