Integrated multiscale analysis reveals complex gender-specific changes in lymphocytes of smokers

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2024-09-10 DOI:10.1016/j.etap.2024.104566

Anne-Cécile Ribou , Florence Riera , Fabienne Durand , Laurent Henry

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引用次数: 0

Abstract

Environmental stressors induce specific physiological responses that can be measured in the blood, notably by morphological changes in lymphocytes. Tobacco being the best-known stress in terms of its impact on health, we studied the physiological properties of peripheral blood lymphocytes in a population of 33 healthy non-smokers and smokers. Proteasome amount, mitochondria energy levels, changes in membrane properties and cell and nuclear size were analyzed to obtain 28 parameters from two fluorescence-based techniques: flow cytometry and cell imaging. The results showed that none of the parameters alone identified gender and smoking status, but that statistical analysis of these parameters, whether or not combined with a third set of data, hematological data, can. Statistical analysis of selected parameters clearly discriminates between male and female samples, as well as smokers and non-smokers. Effects of tobacco smoke pollutants are more pronounced in female smokers than in other groups.

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综合多尺度分析揭示了吸烟者淋巴细胞中复杂的性别特异性变化

环境压力会诱发特定的生理反应，这些反应可以通过血液，特别是淋巴细胞的形态变化来测量。烟草是对健康影响最大的压力，因此我们对 33 名健康的非吸烟者和吸烟者的外周血淋巴细胞的生理特性进行了研究。通过流式细胞仪和细胞成像这两种荧光技术，我们分析了蛋白酶体数量、线粒体能量水平、膜特性变化以及细胞和核大小等 28 个参数。结果表明，没有一个参数能单独识别性别和吸烟状况，但对这些参数进行统计分析，无论是否结合第三组数据（血液学数据），都能识别性别和吸烟状况。对所选参数的统计分析可以明确区分男性和女性样本，以及吸烟者和非吸烟者。与其他群体相比，烟草烟雾污染物对女性吸烟者的影响更为明显。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.