Erika J Félix Santiago, Delia X Vega Manríquez, Milagros González Hernández, Jorge Flores Sánchez, Andrea García Mendoza, Cesar A Rosales Nieto
{"title":"504 Relationship between vaginal microbiota and reproductive efficiency in dairy cows","authors":"Erika J Félix Santiago, Delia X Vega Manríquez, Milagros González Hernández, Jorge Flores Sánchez, Andrea García Mendoza, Cesar A Rosales Nieto","doi":"10.1093/jas/skae234.170","DOIUrl":null,"url":null,"abstract":"Intrinsic and extrinsic factors determine the milk production of a dairy herd, which is affected by the reproductive efficiency of dairy farms. Infectious, nutritional, and management causes can negatively affect this efficiency. After parturition, dairy cows are sensible to clinical diseases since the physical barriers of the cervix, vagina, and vulva are compromised, allowing environmental bacteria to ascend, altering the vaginal microbiota. It is possible that the alteration of the microorganisms of the vaginal microbiota alters the response of circulating hormones related to a new conception and leads to repeat breeder cow syndrome. Repeat breeder cow (RBC) syndrome is considered when the cows have difficulties becoming pregnant after three or more attempts. This was investigated by associating the microorganisms of the vaginal microbiota with the circulating concentration of progesterone, glucose, and reproductive success in Holstein cows. We used 30 Holstein cows of similar body condition and parity (4th) assigned to two treatments based on their historical reproductive success. T-RBC with repeat breeder cow syndrome (n = 15), and T-control (CTL) without (n = 15). At insemination, we collected a vaginal mucosa sample from each cow. The samples were plated on blood agar and McConkey media under aerobiosis and micro-aerobiosis conditions. The isolated colonies were identified through Gram staining and specific biochemical tests to determine the genus and species of each bacterium. Blood samples were collected at insemination, d 5 post insemination, and every 2 d afterward for progesterone and glucose assay. Data for fertility were analyzed using PROC-GLIMMIX with a binomial distribution and logit link function. Data for progesterone and glucose were analyzed using MIXED effects and repetitive measures. Up to date, Bacillus, E coli, Staphylococcus, Klebsiella, Proteus, Streptococcus, and Actinomyces have been isolated. Over time, circulating progesterone and glucose did not differ between treatments (P > 0.05). To date, the fertility rate is similar between treatments (33%; P > 0.05); however, the number of services per conception differed (T-RBC:6 vs T-CTL:1; P < 0.001). The incidence of Bacillus (31% vs 7%; P < 0.001), E. coli (45% vs 4%; P < 0.001), and Staphylococcus (23% vs 9%; P < 0.01) was greater in non-pregnant cows than in pregnant cows. The incidence of the rest of the isolated bacteria was similar between pregnant and non-pregnant cows (P > 0.05). This study suggests that the vaginal microbiota in dairy cows can change according to the reproductive state of the cow. Our study was based on fourth-parity cows; however, more research is needed to elucidate if the same condition is presented in cows with different parity.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of animal science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jas/skae234.170","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Intrinsic and extrinsic factors determine the milk production of a dairy herd, which is affected by the reproductive efficiency of dairy farms. Infectious, nutritional, and management causes can negatively affect this efficiency. After parturition, dairy cows are sensible to clinical diseases since the physical barriers of the cervix, vagina, and vulva are compromised, allowing environmental bacteria to ascend, altering the vaginal microbiota. It is possible that the alteration of the microorganisms of the vaginal microbiota alters the response of circulating hormones related to a new conception and leads to repeat breeder cow syndrome. Repeat breeder cow (RBC) syndrome is considered when the cows have difficulties becoming pregnant after three or more attempts. This was investigated by associating the microorganisms of the vaginal microbiota with the circulating concentration of progesterone, glucose, and reproductive success in Holstein cows. We used 30 Holstein cows of similar body condition and parity (4th) assigned to two treatments based on their historical reproductive success. T-RBC with repeat breeder cow syndrome (n = 15), and T-control (CTL) without (n = 15). At insemination, we collected a vaginal mucosa sample from each cow. The samples were plated on blood agar and McConkey media under aerobiosis and micro-aerobiosis conditions. The isolated colonies were identified through Gram staining and specific biochemical tests to determine the genus and species of each bacterium. Blood samples were collected at insemination, d 5 post insemination, and every 2 d afterward for progesterone and glucose assay. Data for fertility were analyzed using PROC-GLIMMIX with a binomial distribution and logit link function. Data for progesterone and glucose were analyzed using MIXED effects and repetitive measures. Up to date, Bacillus, E coli, Staphylococcus, Klebsiella, Proteus, Streptococcus, and Actinomyces have been isolated. Over time, circulating progesterone and glucose did not differ between treatments (P > 0.05). To date, the fertility rate is similar between treatments (33%; P > 0.05); however, the number of services per conception differed (T-RBC:6 vs T-CTL:1; P < 0.001). The incidence of Bacillus (31% vs 7%; P < 0.001), E. coli (45% vs 4%; P < 0.001), and Staphylococcus (23% vs 9%; P < 0.01) was greater in non-pregnant cows than in pregnant cows. The incidence of the rest of the isolated bacteria was similar between pregnant and non-pregnant cows (P > 0.05). This study suggests that the vaginal microbiota in dairy cows can change according to the reproductive state of the cow. Our study was based on fourth-parity cows; however, more research is needed to elucidate if the same condition is presented in cows with different parity.
期刊介绍:
The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year.
Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.