504 Relationship between vaginal microbiota and reproductive efficiency in dairy cows

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Erika J Félix Santiago, Delia X Vega Manríquez, Milagros González Hernández, Jorge Flores Sánchez, Andrea García Mendoza, Cesar A Rosales Nieto
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引用次数: 0

Abstract

Intrinsic and extrinsic factors determine the milk production of a dairy herd, which is affected by the reproductive efficiency of dairy farms. Infectious, nutritional, and management causes can negatively affect this efficiency. After parturition, dairy cows are sensible to clinical diseases since the physical barriers of the cervix, vagina, and vulva are compromised, allowing environmental bacteria to ascend, altering the vaginal microbiota. It is possible that the alteration of the microorganisms of the vaginal microbiota alters the response of circulating hormones related to a new conception and leads to repeat breeder cow syndrome. Repeat breeder cow (RBC) syndrome is considered when the cows have difficulties becoming pregnant after three or more attempts. This was investigated by associating the microorganisms of the vaginal microbiota with the circulating concentration of progesterone, glucose, and reproductive success in Holstein cows. We used 30 Holstein cows of similar body condition and parity (4th) assigned to two treatments based on their historical reproductive success. T-RBC with repeat breeder cow syndrome (n = 15), and T-control (CTL) without (n = 15). At insemination, we collected a vaginal mucosa sample from each cow. The samples were plated on blood agar and McConkey media under aerobiosis and micro-aerobiosis conditions. The isolated colonies were identified through Gram staining and specific biochemical tests to determine the genus and species of each bacterium. Blood samples were collected at insemination, d 5 post insemination, and every 2 d afterward for progesterone and glucose assay. Data for fertility were analyzed using PROC-GLIMMIX with a binomial distribution and logit link function. Data for progesterone and glucose were analyzed using MIXED effects and repetitive measures. Up to date, Bacillus, E coli, Staphylococcus, Klebsiella, Proteus, Streptococcus, and Actinomyces have been isolated. Over time, circulating progesterone and glucose did not differ between treatments (P > 0.05). To date, the fertility rate is similar between treatments (33%; P > 0.05); however, the number of services per conception differed (T-RBC:6 vs T-CTL:1; P < 0.001). The incidence of Bacillus (31% vs 7%; P < 0.001), E. coli (45% vs 4%; P < 0.001), and Staphylococcus (23% vs 9%; P < 0.01) was greater in non-pregnant cows than in pregnant cows. The incidence of the rest of the isolated bacteria was similar between pregnant and non-pregnant cows (P > 0.05). This study suggests that the vaginal microbiota in dairy cows can change according to the reproductive state of the cow. Our study was based on fourth-parity cows; however, more research is needed to elucidate if the same condition is presented in cows with different parity.
504 奶牛阴道微生物群与繁殖效率的关系
内在和外在因素决定着奶牛群的产奶量,而奶牛群的产奶量又受奶牛场繁殖效率的影响。感染、营养和管理等原因都会对这种效率产生负面影响。分娩后,奶牛容易感染临床疾病,因为子宫颈、阴道和外阴的物理屏障受到破坏,环境细菌得以进入,从而改变了阴道微生物群。阴道微生物群的改变可能会改变与新受孕有关的循环激素的反应,从而导致重复配种母牛综合症。重复配种母牛(RBC)综合征是指母牛在三次或三次以上尝试后仍难以怀孕。我们通过将荷斯坦奶牛阴道微生物群的微生物与循环中的孕酮浓度、葡萄糖和繁殖成功率联系起来进行了研究。我们使用了 30 头体况和奇数(第 4 奇数)相似的荷斯坦奶牛,根据其历史繁殖成功率将其分配到两种处理中。患有重复育种牛综合症的 T-RBC(n = 15)和不患有重复育种牛综合症的 T-对照(CTL)(n = 15)。授精时,我们采集了每头奶牛的阴道粘膜样本。在无菌和微无菌条件下,将样本培养在血琼脂和麦康凯培养基上。分离出的菌落通过革兰氏染色和特定生化测试进行鉴定,以确定每种细菌的属和种。在授精时、授精后第 5 天以及之后每隔 2 天采集血样,进行孕酮和葡萄糖检测。生育力数据采用 PROC-GLIMMIX 方法进行分析,该方法采用二项分布和 logit 连接功能。孕酮和葡萄糖的数据采用 MIXED 效果和重复测量法进行分析。迄今为止,已分离出芽孢杆菌、大肠杆菌、葡萄球菌、克雷伯氏菌、变形杆菌、链球菌和放线菌。随着时间的推移,循环孕酮和葡萄糖在不同处理之间没有差异(P > 0.05)。迄今为止,不同治疗方法的受孕率相似(33%;P > 0.05);但每次受孕的服务次数不同(T-RBC:6 vs T-CTL:1;P < 0.001)。非怀孕母牛的芽孢杆菌(31% vs 7%;Pamp;lt; 0.001)、大肠杆菌(45% vs 4%;Pamp;lt; 0.001)和葡萄球菌(23% vs 9%;Pamp;lt; 0.01)发病率高于怀孕母牛。其他分离细菌的发生率在妊娠奶牛和非妊娠奶牛中相似(P> 0.05)。这项研究表明,奶牛阴道微生物群会随着奶牛的繁殖状态而变化。我们的研究基于四胎奶牛,但还需要更多的研究来阐明不同胎次的奶牛是否会出现同样的情况。
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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