PSI-13 The impact of divergent nutritional planes on systemic immune status in mature bulls

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2024-09-14 DOI:10.1093/jas/skae234.533

Holly E Hamilton, Elizabeth Shepherd, Saulo M Zoca, Carl R Dahlen, Alexandria P Snider, Kyle J McLean

{"title":"PSI-13 The impact of divergent nutritional planes on systemic immune status in mature bulls","authors":"Holly E Hamilton, Elizabeth Shepherd, Saulo M Zoca, Carl R Dahlen, Alexandria P Snider, Kyle J McLean","doi":"10.1093/jas/skae234.533","DOIUrl":null,"url":null,"abstract":"Bull fertility is a major component of reproductive efficiency. One of the many factors influencing reproductive efficiency in bulls is nutrition. Improper nutrition influences physiological features, such as body weight (BW) and body condition scores (BCS), but also increases stress, which can alter the molecular composition of ejaculate. Following a breeding soundness exam and a 21-d dietary adjustment period, mature Angus bulls (n = 6) were randomly assigned to one of two nutritional planes for a 119-d period: 1) restriction (RES; targeted a 1.36 kg/d decrease in BW) or 2) maintenance (MNT; targeted to maintain BW). Body weight and BCS were evaluated every 14 d and feed amounts were adjusted to meet BW targets. On d 73 and 93, scrotal circumference and blood samples were collected for analysis. Serum samples were evaluated for an immunity score using the Nano Discovery D2Dx Immunity Test, that assesses samples for the presence of immunoglobulin (Ig) G, IgM, cytokines, and complement. Statistical analyses were conducted using R Studio to determine the effects of day, nutritional plane, and the interaction of day Î nutritional plane on BW, BCS, scrotal circumference, and systemic immunity score. At the onset of treatments, all bulls had a greater (P = 0.008) BW (845.5 ± 17.0 kg) and BCS (6.3 ± 0.15) than on d 73 (BW = 757.2 ± 17.0 kg; BCS = 5.5 ± 0.15, respectively) or 93 (BW = 749.6 ± 17.0 kg; BCS = 5.3 ± 0.15, respectively). According to the experimental model design, the MNT bulls had a greater (P = 0.001) BCS (6.1 ± 0.12) compared with the RES bulls (5.3 ± 0.12) throughout treatment. Body weights were also influenced by treatment with MNT bulls (845.7 ± 13.9 kg) having a greater BW (P &gt; 0.001) compared with RES bulls (714.6 ± 13.9). Immunity scores were greater in the RES bulls (0.03 ± 0.003) compared with MNT bulls (0.003 ± 0.003). However, there was no effect of day or the interaction effect (P &gt; 0.05) on the systemic immunity score. There was also no effect of day, nutritional plane, or interaction on scrotal circumference (P &gt; 0.23). In conclusion, we observed nutritional planes to result in divergent BW and BCS and were influential on systemic immunity scores, which may lead to different seminal plasma compositions and reproductive outcomes. Further analyses are needed to elucidate the effects of paternal nutritional stress on the female uterine response after mating and reproductive efficiency. The authors would like to thank USDA-NIFA-AFRI (#2023-67015-39453) and USDA-NIFA Multi-State Project No. 1019048 for providing funding and support for this research. USDA is an equal opportunity employer and provider.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of animal science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jas/skae234.533","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Bull fertility is a major component of reproductive efficiency. One of the many factors influencing reproductive efficiency in bulls is nutrition. Improper nutrition influences physiological features, such as body weight (BW) and body condition scores (BCS), but also increases stress, which can alter the molecular composition of ejaculate. Following a breeding soundness exam and a 21-d dietary adjustment period, mature Angus bulls (n = 6) were randomly assigned to one of two nutritional planes for a 119-d period: 1) restriction (RES; targeted a 1.36 kg/d decrease in BW) or 2) maintenance (MNT; targeted to maintain BW). Body weight and BCS were evaluated every 14 d and feed amounts were adjusted to meet BW targets. On d 73 and 93, scrotal circumference and blood samples were collected for analysis. Serum samples were evaluated for an immunity score using the Nano Discovery D2Dx Immunity Test, that assesses samples for the presence of immunoglobulin (Ig) G, IgM, cytokines, and complement. Statistical analyses were conducted using R Studio to determine the effects of day, nutritional plane, and the interaction of day Î nutritional plane on BW, BCS, scrotal circumference, and systemic immunity score. At the onset of treatments, all bulls had a greater (P = 0.008) BW (845.5 ± 17.0 kg) and BCS (6.3 ± 0.15) than on d 73 (BW = 757.2 ± 17.0 kg; BCS = 5.5 ± 0.15, respectively) or 93 (BW = 749.6 ± 17.0 kg; BCS = 5.3 ± 0.15, respectively). According to the experimental model design, the MNT bulls had a greater (P = 0.001) BCS (6.1 ± 0.12) compared with the RES bulls (5.3 ± 0.12) throughout treatment. Body weights were also influenced by treatment with MNT bulls (845.7 ± 13.9 kg) having a greater BW (P > 0.001) compared with RES bulls (714.6 ± 13.9). Immunity scores were greater in the RES bulls (0.03 ± 0.003) compared with MNT bulls (0.003 ± 0.003). However, there was no effect of day or the interaction effect (P > 0.05) on the systemic immunity score. There was also no effect of day, nutritional plane, or interaction on scrotal circumference (P > 0.23). In conclusion, we observed nutritional planes to result in divergent BW and BCS and were influential on systemic immunity scores, which may lead to different seminal plasma compositions and reproductive outcomes. Further analyses are needed to elucidate the effects of paternal nutritional stress on the female uterine response after mating and reproductive efficiency. The authors would like to thank USDA-NIFA-AFRI (#2023-67015-39453) and USDA-NIFA Multi-State Project No. 1019048 for providing funding and support for this research. USDA is an equal opportunity employer and provider.

查看原文本刊更多论文

PSI-13 不同营养方案对成年公牛全身免疫状态的影响

公牛的繁殖力是繁殖效率的重要组成部分。营养是影响公牛繁殖效率的众多因素之一。营养不良会影响生理特征，如体重（BW）和体况评分（BCS），还会增加应激，从而改变射精的分子组成。经过育种健康检查和 21 天的饮食调整期后，成年安格斯公牛（n = 6）被随机分配到两种营养方案中的一种，为期 119 天：1）限制（RES；目标是体重每天减少 1.36 千克）或 2）维持（MNT；目标是维持体重）。每 14 天对体重和 BCS 进行一次评估，并调整饲料量以达到体重目标。第 73 天和第 93 天，收集阴囊周长和血液样本进行分析。使用 Nano Discovery D2Dx 免疫测试对血清样本进行免疫评分评估，该测试可评估样本中是否存在免疫球蛋白 (Ig)G、IgM、细胞因子和补体。使用 R Studio 进行了统计分析，以确定日龄、营养平面以及日龄 Î 营养平面的交互作用对体重、BCS、阴囊周长和全身免疫力评分的影响。与第 73 天（BW = 757.2 ± 17.0 kg；BCS = 5.5 ± 0.15，分别）或第 93 天（BW = 749.6 ± 17.0 kg；BCS = 5.3 ± 0.15，分别）相比，所有公牛的体重（845.5 ± 17.0 kg）和 BCS（6.3 ± 0.15）在处理开始时都较大（P = 0.008）。根据实验模型设计，在整个处理过程中，MNT 公牛的 BCS（6.1 ± 0.12）高于 RES 公牛（5.3 ± 0.12）（P = 0.001）。体重也受到治疗的影响，MNT 公牛的体重（845.7 ± 13.9 千克）比 RES 公牛的体重（714.6 ± 13.9）大（P > 0.001）。与 MNT 公牛（0.003 ± 0.003）相比，RES 公牛的免疫评分更高（0.03 ± 0.003）。然而，天数或交互效应（P > 0.05）对全身免疫得分没有影响。日、营养平面或交互作用对阴囊周长也没有影响（P&;gt; 0.23）。总之，我们观察到营养平面导致不同的体重和BCS，并对全身免疫力评分有影响，这可能导致不同的精浆成分和繁殖结果。要阐明父亲营养应激对交配后雌性子宫反应和繁殖效率的影响，还需要进一步的分析。作者感谢 USDA-NIFA-AFRI (#2023-67015-39453) 和 USDA-NIFA 多州项目 1019048 为本研究提供资金和支持。美国农业部是一个机会均等的雇主和供应商。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.