PSIV-10 Thyroid hormone does not protect skeletal muscle mitochondrial function from heat stress in pigs

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of animal science Pub Date : 2024-09-14 DOI:10.1093/jas/skae234.614

Lauren T Wesolowski, Jamianne J Kelly, Melissa S Roths, Tori E Rudolph, Pier L Semanchik, Robert P Rhoads, Lance H Baumgard, Joshua T Selsby, Sarah H White-Springer

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Abstract

Environmental heat stress (HS) can impair muscle mitochondrial function which may contribute to negative health outcomes, but little is known about potential treatments to rescue mitochondrial function during HS. To test the hypothesis that thyroid hormone (T3) would protect mitochondrial function during HS, samples were collected from the right ventricle (RV) of 3-mo-old, crossbred gilts under 4 different environmental conditions: thermoneutral (TN; 22°C; n = 8); 1 d HS (HS1; 38°C; n = 7); oral supplementation of 1.33 µgּ kg body weight (BW)-1ּ d-1 Cytomel (liothyronine sodium) during the 1 d HS (HS1TH; n = 8); and 7 d HS (HS7; n = 8). The oxidative portion of the semitendinosus (ST) was also collected from all groups plus a 5th group: oral supplementation of Cytomel during 7 d HS (HS7TH, n = 8). Mitochondrial volume density was estimated by citrate synthase (CS) activity, and oxidative phosphorylation (OP) and electron transfer (E) capacities were determined via high resolution respirometry. Data were analyzed using mixed linear models in SAS v9.4 with treatment group as a fixed effect and pig (treatment) as a random effect. Within the RV, the contribution of OP supported by complex I (PCI) to maximal E was less in HS1TH gilts compared with all other groups (P ≤ 0.02), and the contribution of E supported by complex II (ECII) to maximal E was greater in TN than HS1 or HS7 (P ≤ 0.02) and tended to be greater in TN compared with HS1TH gilts (P = 0.07). Within the ST, integrative (relative to tissue wet weight) PCI was greatest in TN gilts (vs. HS7 and HS7TH, P ≤ 0.0002; vs. HS1 and HS1TH, P ≤ 0.06) and least in HS7 and HS7TH (vs. HS1 and HS1TH, P ≤ 0.05). Integrative maximal P (PCI+II) was greatest in TN pigs (compared with HS1TH, HS7, and HS7TH, P ≤ 0.05; compared with HS1, P = 0.09). Integrative maximal E (ECI+II) was also greatest in TN gilts than all other groups (P ≤ 0.05) while ECII was greater in TN pigs compared with HS7 and HS7TH (P ≤ 0.009) and tended to be greater than HS1 and HS1TH (P ≤ 0.09). In ST, intrinsic (relative to CS activity) PCI and PCI+II were greater in TN compared with HS1, HS1TH, and HS7 pigs (P ≤ 0.03) and PCI+II tended to be greater in TN than HS7TH (P ≤ 0.06). Intrinsic ECI+II and ECII were greater in TN compared with all other treatment groups (P ≤ 0.04). These results suggest that T3 supplementation did not protect mitochondrial function from negative impacts of HS in skeletal muscle but may have modified electron transfer through complex I in cardiac muscle which may have implications on oxidative stress.

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PSIV-10 甲状腺激素不能保护猪骨骼肌线粒体功能免受热应激影响

环境热应激（HS）会损害肌肉线粒体功能，从而导致不良的健康后果，但人们对在HS期间挽救线粒体功能的潜在治疗方法知之甚少。为了验证甲状腺激素（T3）能在 HS 期间保护线粒体功能的假设，我们从 3 月龄杂交后备母猪的右心室（RV）采集了样本，在 4 种不同的环境条件下进行了试验：中温（TN；22°C；n = 8）；1 d HS（HS1；38°C；n = 7）；口服补充 1.33 µgּ kg 体重 (BW)-1ּ d-1 Cytomel（甲状腺素钠）。此外，还从所有组别和第 5 组别中收集了半腱肌 (ST) 的氧化部分：在 7 d HS（HS7TH，n = 8）期间口服 Cytomel 补充剂。线粒体体积密度通过柠檬酸合成酶（CS）活性估算，氧化磷酸化（OP）和电子传递（E）能力通过高分辨率呼吸测定法确定。数据采用 SAS v9.4 混合线性模型进行分析，处理组为固定效应，猪（处理）为随机效应。在RV中，与所有其他组相比，HS1TH后备母猪由复合体I（PCI）支持的OP对最大E的贡献较小（P≤0.02），而由复合体II（ECII）支持的E对最大E的贡献在TN中大于HS1或HS7（P≤0.02），并且与HS1TH后备母猪相比，TN中的贡献往往更大（P = 0.07）。在ST内，TN后备母猪的整合性（相对于组织湿重）PCI最大（与HS7和HS7TH相比，P≤0.0002；与HS1和HS1TH相比，P≤0.06），而HS7和HS7TH最小（与HS1和HS1TH相比，P≤0.05）。TN 猪的整合最大 P（PCI+II）最大（与 HS1TH、HS7 和 HS7TH 相比，P ≤ 0.05；与 HS1 相比，P = 0.09）。TN后备母猪的整合最大E（ECI+II）也比其他所有组别都大（P≤0.05），而与HS7和HS7TH相比，TN猪的ECII更大（P≤0.009），且往往比HS1和HS1TH更大（P≤0.09）。在 ST 中，与 HS1、HS1TH 和 HS7 猪相比，TN 猪的内在（相对于 CS 活性）PCI 和 PCI+II 更大（P≤ 0.03），TN 猪的 PCI+II 往往比 HS7TH 大（P≤ 0.06）。与所有其他治疗组相比，TN 组的内在 ECI+II 和 ECII 更大（P ≤ 0.04）。这些结果表明，补充 T3 并不能保护骨骼肌线粒体功能免受 HS 的负面影响，但可能改变了心肌中通过复合体 I 的电子传递，这可能对氧化应激产生影响。

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来源期刊

Journal of animal science 农林科学-奶制品与动物科学

CiteScore

4.80

自引率

12.10%

发文量

1589

审稿时长

3 months

期刊介绍： The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.