Proangiogenic potential of plasma exosomes from prostate cancer patients

IF 4.4 2区 生物学 Q2 CELL BIOLOGY
Chao Wang , Chuan Zhou , Dong Wang , Yun-Feng Zhang , Hao-Xuan Lv , Han He , Yong-qi Ren , Jia Wang , Feng-Hai Zhou
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引用次数: 0

Abstract

Angiogenesis plays a pivotal role in the progression and metastasis of solid cancers, including prostate cancer (PCa). While small extracellular vesicles derived from PCa cell lines induce a proangiogenic phenotype in vascular endothelial cells, the contribution of plasma exosomes from patients with PCa to this process remains unclear. Here, we successfully extracted and characterized plasma exosomes. Notably, a ring of PKH67-labeled exosomes was observed around the HUVEC nucleus using fluorescence microscopy, indicating the uptake of exosomes by HUVEC. At the cellular level, PCa plasma exosomes enhanced angiogenesis, proliferation, invasion, and migration of HUVEC cells. Moreover, PCa plasma exosomes promoted angiogenesis and aortic sprouting. MicroRNAs are the most common genetic material in exosomes, and to identify miRNAs associated with the angiogenic response, we performed small RNA sequencing followed by RT-qPCR and bioinformatics analysis. These analyses revealed distinct miRNA profiles in plasma exosomes from patients with PCa compared to healthy individuals. Notably, hsa-miR-184 emerged as a potential regulator implicated in the proangiogenic effects of PCa plasma exosomes.

前列腺癌患者血浆外泌体的促血管生成潜力
血管生成在包括前列腺癌(PCa)在内的实体癌的进展和转移过程中起着关键作用。虽然从 PCa 细胞系中提取的小细胞外囊泡能诱导血管内皮细胞的促血管生成表型,但 PCa 患者的血浆外泌体对这一过程的贡献仍不清楚。在这里,我们成功提取并鉴定了血浆外泌体。值得注意的是,用荧光显微镜观察到HUVEC细胞核周围有一圈PKH67标记的外泌体,这表明HUVEC吸收了外泌体。在细胞水平上,PCa 血浆外泌体增强了 HUVEC 细胞的血管生成、增殖、侵袭和迁移。此外,PCa 血浆外泌体还能促进血管生成和主动脉萌芽。微RNA是外泌体中最常见的遗传物质,为了确定与血管生成反应相关的miRNA,我们进行了小RNA测序,然后进行了RT-qPCR和生物信息学分析。这些分析表明,与健康人相比,PCa 患者血浆外泌体中的 miRNA 特征截然不同。值得注意的是,hsa-miR-184 是与 PCa 血浆外泌体的促血管生成作用有关的潜在调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cellular signalling
Cellular signalling 生物-细胞生物学
CiteScore
8.40
自引率
0.00%
发文量
250
审稿时长
27 days
期刊介绍: Cellular Signalling publishes original research describing fundamental and clinical findings on the mechanisms, actions and structural components of cellular signalling systems in vitro and in vivo. Cellular Signalling aims at full length research papers defining signalling systems ranging from microorganisms to cells, tissues and higher organisms.
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