Functional characterization of a small gene family coding for putrescine hydroxycinnamoyltransferases, involved in phenolamide accumulation, in tomato

IF 3.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2024-09-12 DOI:10.1016/j.phytochem.2024.114271

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Abstract

Phenolamides are specialized metabolites widely distributed in the plant kingdom. Their structure is composed by the association of hydroxycinnamic acid derivatives to mono-/poly-amine through an amination catalyzed by N-hydroxycinnamoyltransferases enzymes. Tomato plants accumulate putrescine-derived phenolamides in their vegetative parts. Recently, two first genes coding for putrescine-hydroxycinnamoyltransferase (PHT, Solyc11g071470 and Solyc11g071480) were identified in tomato and demonstrated to control the leaf accumulation of caffeoylputrescine in response to leafminer infestation. In this study, two additional genes (Solyc06g074710 and Solyc11g066640) were functionally characterized as new tomato PHT. The substrate specificity and the expression pattern in planta were determined for the four tomato PHT. Taken together the results give a comprehensive view of the control of the putrescine-derived phenolamide accumulation in tomato plant through the biochemical specificity and the spatial expression of this small family of PHT.

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番茄中参与苯甲酰胺积累的腐胺羟基肉桂酰转移酶编码小基因家族的功能表征

酚酰胺是一种特殊的代谢产物，广泛分布于植物界。其结构由羟基肉桂酸衍生物通过 N-羟基肉桂酰转移酶催化的胺化作用与单胺/多胺结合而成。番茄植株的无性部分会积累由腐胺衍生的酚酰胺。最近，在番茄中首次发现了两个编码腐胺-羟基肉桂酰基转移酶（PHT，Solyc11g071470 和 Solyc11g071480）的基因，并证明这两个基因能控制叶片中咖啡酰基腐胺的积累，以应对潜叶蝇的侵袭。在本研究中，另外两个基因（Solyc06g074710 和 Solyc11g066640）被鉴定为新的番茄 PHT。研究还确定了这四个番茄 PHT 的底物特异性和在植物体内的表达模式。总之，这些结果全面揭示了番茄植物中通过 PHT 小家族的生化特异性和空间表达控制腐胺衍生的苯甲酰胺积累的过程。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.