Intrinsic physiochemical insights to green synthesized Ag-decorated GO nanosheet for photoluminescence and in vivo cellular biocompatibility with embryonic zebrafish

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-09-06 DOI:10.1016/j.colsurfb.2024.114212

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引用次数: 0

Abstract

The advancement of nanotechnology and their application has intrigued a significant interest in green synthesis and application of organic and inorganic nanomaterials like graphene oxide (GO) and silver nanoparticles (AgNP). This study explores the intrinsic physiochemical properties of silver (Ag)-decorated graphene oxide (GO) nanosheets synthesized via a green approach, focusing on their photoluminescence behaviour and in vivo cellular biocompatibility with embryonic zebrafish. The nanocomposites were characterized using various spectroscopic and microscopic techniques to elucidate their structural and optical properties. Results reveal that the Ag-decorated GO nanosheets exhibit enhanced photoluminescence compared to pristine GO with an SPR at 405 nm and emission at 676 nm, attributed to the synergistic effects of Ag nanoparticles and GO. In addition, in vivo biocompatibility assessments using embryonic zebrafish demonstrate minimal cytotoxicity and high cellular viability upon exposure to the nanocomposites with an LC50 of 38 µg/ml, indicating their potential for biomedical applications. Further investigations into the interactions between the nanomaterials and biological systems provide valuable insights into their safety profile and suggest their suitability for various biomedical and therapeutic applications. Overall, this study offers a comprehensive understanding of the physiochemical characteristics and biological compatibility of Ag-decorated GO nanosheets, contributing to the advancement of nanotechnology in biomedicine and related fields.

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绿色合成的银饰 GO 纳米片的光致发光和与胚胎斑马鱼体内细胞生物相容性的内在物理化学见解

纳米技术的发展及其应用引起了人们对氧化石墨烯（GO）和银纳米粒子（AgNP）等有机和无机纳米材料的绿色合成和应用的极大兴趣。本研究探索了通过绿色方法合成的银（Ag）装饰氧化石墨烯（GO）纳米片的内在理化特性，重点研究了它们的光致发光行为以及与胚胎斑马鱼的体内细胞生物相容性。利用各种光谱和显微技术对纳米复合材料进行了表征，以阐明其结构和光学特性。结果表明，与原始 GO 相比，Ag 装饰的 GO 纳米片具有更强的光致发光性能，其 SPR 波长为 405 nm，发射波长为 676 nm，这归因于 Ag 纳米粒子和 GO 的协同效应。此外，利用胚胎斑马鱼进行的体内生物相容性评估表明，纳米复合材料的细胞毒性极小，细胞存活率高，半数致死浓度为 38 µg/ml，这表明它们具有生物医学应用潜力。对纳米材料与生物系统之间相互作用的进一步研究为了解纳米材料的安全性提供了宝贵的信息，并表明它们适合用于各种生物医学和治疗应用。总之，本研究全面了解了银饰 GO 纳米片的理化特性和生物相容性，有助于推动纳米技术在生物医学及相关领域的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.