Influence of carbon to phosphorus ratio on the performance of single-stage aerobic simultaneous nitrogen and phosphorus removal by bioflocs

IF 3.6 2区 农林科学 Q2 AGRICULTURAL ENGINEERING
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Abstract

Effluents from intensive aquaculture typically contain high nitrate and phosphate concentrations. Biofloc technology has demonstrated the potential for simultaneous removal of nitrate and phosphate without ammonium nitrogen, and further optimization is needed to enhance the nitrogen and phosphorus removal efficiency. In this study, we investigated the efficiency of bioflocs in treating highly concentrated aquacultural wastewater at different carbon to phosphorus (C/P) ratios of 20 (G20), 30 (G30), and 40 (G40). The results showed that the nitrate removal rate in group G40 (1.25±0.07 mgN/gTSS/h) was significantly higher than in groups G20 and G30 (p < 0.05). However, there was no significant difference between the phosphate removal rates of groups G40 and G30, while both exhibited superior G20. The relative abundance of Thauera in G40 was significantly higher (p < 0.05), accounting for 8.74 % of the microbial community. Additionally, the copy counts of denitrification-related genes (napA, nirS, nirK, nosZ) and inorganic phosphate transport genes (pqqC) were significantly higher in G40, correlating positively with an increased C/P ratio. These results suggest that the excessive carbon source in G40 enhanced denitrification and reduced biofloc assimilation, thus failing to significantly enhance the phosphate removal rate. This study demonstrates that adjusting the C/P ratio alone can improve the efficiency of nitrogen and phosphorus removal by bioflocs, and that a C/P ratio of 30 may be the most appropriate for enhancing the rate of nutrient removal while minimizing the use of carbon source.

碳磷比对生物絮体单级好氧同时脱氮除磷性能的影响
集约化水产养殖产生的废水通常含有较高浓度的硝酸盐和磷酸盐。生物絮凝技术已证明具有同时去除硝酸盐和磷酸盐而不含铵态氮的潜力,但仍需进一步优化以提高脱氮除磷效率。在这项研究中,我们考察了生物絮凝体在不同碳磷(C/P)比(20(G20)、30(G30)和 40(G40))条件下处理高浓度水产养殖废水的效率。结果表明,G40 组的硝酸盐去除率(1.25±0.07 mgN/gTSS/h)明显高于 G20 和 G30 组(p <0.05)。然而,G40 组和 G30 组的磷酸盐去除率没有明显差异,而 G20 组和 G30 组的磷酸盐去除率均优于 G20 组。G40 中 Thauera 的相对丰度明显更高(p < 0.05),占微生物群落的 8.74%。此外,反硝化相关基因(napA、nirS、nirK、nosZ)和无机磷酸盐转运基因(pqqC)的拷贝数在 G40 中也明显较高,这与 C/P 比值的增加呈正相关。这些结果表明,G40 中过多的碳源增强了反硝化作用,降低了生物絮凝物同化作用,从而未能显著提高磷酸盐去除率。这项研究表明,仅调整 C/P 比值就能提高生物絮体去除氮和磷的效率,C/P 比值为 30 可能最适合提高养分去除率,同时最大限度地减少碳源的使用。
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来源期刊
Aquacultural Engineering
Aquacultural Engineering 农林科学-农业工程
CiteScore
8.60
自引率
10.00%
发文量
63
审稿时长
>24 weeks
期刊介绍: Aquacultural Engineering is concerned with the design and development of effective aquacultural systems for marine and freshwater facilities. The journal aims to apply the knowledge gained from basic research which potentially can be translated into commercial operations. Problems of scale-up and application of research data involve many parameters, both physical and biological, making it difficult to anticipate the interaction between the unit processes and the cultured animals. Aquacultural Engineering aims to develop this bioengineering interface for aquaculture and welcomes contributions in the following areas: – Engineering and design of aquaculture facilities – Engineering-based research studies – Construction experience and techniques – In-service experience, commissioning, operation – Materials selection and their uses – Quantification of biological data and constraints
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