A pilot study of intestinal protist detection in humans, animals, and the environment in a slum area in Mymensingh, Bangladesh

IF 1.5 4区医学 Q3 PARASITOLOGY

Parasitology International Pub Date : 2024-09-11 DOI:10.1016/j.parint.2024.102967

Sadiya Maxamhud , Md Shahiduzzaman , A.R.M. Beni Amin , Md. Zawad Hossain , Eleni Gentekaki , Anastasios D. Tsaousis

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Abstract

The purpose of this study was to investigate the presence and transmission of Cryptosporidium spp., Blastocystis sp., Giardia intestinalis and Entamoeba histolytica between different hosts and their shared environment in a slum area of Bangladesh. A total of 102 samples were collected from Mymensingh, Bangladesh. This collection encompassed 16 human samples, 35 soil samples and 51 animal samples from various households within the region. The detection of Cryptosporidium spp., Blastocystis sp., G. intestinalis, and E. histolytica was carried out using nested PCR and/or quantitative PCR methodologies. Among the samples, 24 human, animal, and soil out of 102 were found positive for Blastocystis sp., spanning seven subtypes: ST2, ST3, ST7, ST10, ST23, ST24 and ST25. Additionally, eight samples (8/102) including human, animal and soil tested positive for Cryptosporidium spp., including C. parvum, C. baileyi, C. bovis, and C. meleagridis. Furthermore, one soil sample tested positive for G. intestinalis assemblage B, while no samples tested positive for E. histolytica. The detection of Cryptosporidium spp., Blastocystis sp., and G. intestinalis in this study has provided insights into their presence, extending beyond humans. Moreover, these findings highlight the importance of embracing a One Health perspective with an emphasis on specific parasitic microorganisms.

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孟加拉国迈门辛贫民窟地区人、动物和环境中肠道原生动物检测试点研究

本研究旨在调查孟加拉国贫民窟地区不同宿主及其共同环境中隐孢子虫属、布氏囊虫属、肠道贾第虫和组织溶解恩塔米巴虫的存在和传播情况。我们从孟加拉国的迈门辛收集了 102 份样本。这些样本包括 16 份人体样本、35 份土壤样本和 51 份动物样本，分别来自该地区的不同家庭。采用巢式 PCR 和/或定量 PCR 方法检测了隐孢子虫属、布氏囊虫属、肠球孢子虫属和组织溶解性大肠杆菌。在 102 份样本中，发现 24 份人体、动物和土壤样本中的 Blastocystis sp：ST2、ST3、ST7、ST10、ST23、ST24 和 ST25。此外，包括人类、动物和土壤在内的 8 份样本（8/102）对隐孢子虫属检测呈阳性，包括 C. parvum、C. baileyi、C. bovis 和 C. meleagridis。此外，有一份土壤样本的肠杆菌 B 组合检测呈阳性，而没有样本的溶组织埃希氏菌检测呈阳性。在这项研究中检测到的隐孢子虫属、布氏囊虫属和肠道球孢子虫使人们对它们的存在有了更深入的了解，并将其范围扩大到人类以外。此外，这些发现还强调了从 "一体健康 "的角度看待特定寄生微生物的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasitology International 医学-寄生虫学

CiteScore

4.00

自引率

10.50%

发文量

140

审稿时长

61 days

期刊介绍： Parasitology International provides a medium for rapid, carefully reviewed publications in the field of human and animal parasitology. Original papers, rapid communications, and original case reports from all geographical areas and covering all parasitological disciplines, including structure, immunology, cell biology, biochemistry, molecular biology, and systematics, may be submitted. Reviews on recent developments are invited regularly, but suggestions in this respect are welcome. Letters to the Editor commenting on any aspect of the Journal are also welcome.