Genetic diversity among Ethiopian sweet sorghum [Sorghum bicolor (L.) Moench] accessions using simple sequence repeat markers

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-08-05 DOI:10.1016/j.genrep.2024.102007

Melkamu Genet , Wosene Gebreselassie , Tsegaye Getahun , Tileye Feyissa

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引用次数: 0

Abstract

Sweet sorghum (Sorghum bicolor (L.) Moench) is the only grain and stalk crop that can be used for multipurpose. Despite its global significance and potential, it faced genetic erosion. These are due to low productivity and lower farmers' preferences, which is not considered one of the most important cereal crops in Ethiopia. To utilize and popularize these crops, understanding the genetic diversity and population structure is a pre-request. Therefore, this study was aimed to assess the genetic diversity and population structure of selected 82 Ethiopian sweet sorghum accessions using 10 simple sequence repeat (SSR) markers that represent seven geographic regions of Ethiopia. The study revealed a total of 116 alleles with a mean of 11.6 alleles per locus. All used microsatellite loci were highly polymorphic with polymorphic information content (PIC) ranging from 0.75 to 0.90 with an average of 0.82. They showed high gene diversity ranging from 0.59 to 0.81 with an overall mean of 0.70. There was a moderate genetic differentiation (FST = 0.21) showing the presence of high gene flow (Nm = 5.033) where 91 % of the total variation was accounted for within populations genetic variability. The clustering, principal coordinate analysis (PCoA) and population structure did not cluster the studied populations into a separate group according to their geographical origin. In conclusion, the highest intra-population diversity was observed among populations of North Wollo (He = 0.81) and South Wollo (He = 0.79), and hence these areas can be considered as hot spots for the identification of novel traits. Therefore, the present study has generated baseline information for breeders to improve Ethiopian sweet sorghum through breeding, management, and conservation of the available genetic resources.

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利用简单序列重复标记研究埃塞俄比亚甜高粱[Sorghum bicolor (L.) Moench]品种的遗传多样性

甜高粱（Sorghum bicolor (L.) Moench）是唯一可用于多种用途的谷物和茎秆作物。尽管甜高粱在全球具有重要意义和潜力，但它却面临着基因侵蚀的问题。其原因是产量低，农民的喜好度也较低，因此并未被视为埃塞俄比亚最重要的谷类作物之一。要利用和推广这些作物，了解其遗传多样性和种群结构是一项先决条件。因此，本研究旨在利用代表埃塞俄比亚七个地理区域的 10 个简单序列重复（SSR）标记，评估选定的 82 个埃塞俄比亚甜高粱品种的遗传多样性和种群结构。研究共发现 116 个等位基因，平均每个位点有 11.6 个等位基因。所有使用的微卫星位点都具有高度多态性，多态信息含量（PIC）在 0.75 至 0.90 之间，平均为 0.82。基因多样性从 0.59 到 0.81 不等，平均值为 0.70。遗传分化程度适中（FST = 0.21），表明存在高基因流（Nm = 5.033），种群内部遗传变异占总变异的 91%。聚类、主坐标分析（PCoA）和种群结构并未根据地理起源将研究种群划分为一个单独的群体。总之，在北沃洛（He = 0.81）和南沃洛（He = 0.79）的种群中观察到了最高的种群内多样性，因此这些地区可被视为鉴定新性状的热点地区。因此，本研究为育种者通过育种、管理和保护现有遗传资源改良埃塞俄比亚甜高粱提供了基础信息。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.