Delivery mode and maternal gestational diabetes are important factors in shaping the neonatal initial gut microbiota

IF 4.6 2区医学 Q2 IMMUNOLOGY

Frontiers in Cellular and Infection Microbiology Pub Date : 2024-08-29 DOI:10.3389/fcimb.2024.1397675

Xuan Shi, Yanfang Liu, Teng Ma, Hao Jin, Feiyan Zhao, Zhihong Sun

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Abstract

BackgroundThe infant gut microbiome’s establishment is pivotal for health and immune development. Understanding it unveils insights into growth, development, and maternal microbial interactions. Research often emphasizes gut bacteria, neglecting the phageome.MethodsTo investigate the influence of geographic or maternal factors (mode of delivery, mode of breastfeeding, gestational diabetes mellitus) on the gut microbiota and phages of newborns, we collected fecal samples from 34 pairs of mothers and their infants within 24 hours of delivery from three regions (9 pairs from Enshi, 7 pairs from Hohhot, and 18 pairs from Hulunbuir) using sterile containers. Gut microbiota analysis by Shotgun sequencing was subsequently performed.ResultsOur results showed that geographic location affects maternal gut microbiology (P < 0.05), while the effect on infant gut microbiology was not significant (P = 0.184). Among the maternal factors, mode of delivery had a significant (P < 0.05) effect on the newborn. Specific bacteria (e.g., Bacteroides, Escherichia spp., Phocaeicola vulgatus, Escherichia coli, Staphylococcus hominis, Veillonella spp.), predicted active metabolites, and bacteriophage vOTUs varied with delivery mode. Phocaeicola vulgatus significantly correlated with some metabolites and bacteriophages in the early infant gut (P < 0.05). In the GD group, a strong negative correlation of phage diversity between mother and infants was observed (R = -0.58, P=0.04).ConclusionIn conclusion, neonatal early gut microbiome (including bacteria and bacteriophages) colonization is profoundly affected by the mode of delivery, and maternal gestational diabetes mellitus. The key bacteria may interact with bacteriophages to influence the levels of specific metabolites. Our study provides new evidence for the study of the infant microbiome, fills a gap in the analysis of the infant gut microbiota regarding the virome, and emphasizes the importance of maternal health for the infant initial gut virome.

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分娩方式和母体妊娠糖尿病是影响新生儿初始肠道微生物群的重要因素

背景婴儿肠道微生物组的建立对健康和免疫发育至关重要。了解婴儿肠道微生物组将有助于深入了解婴儿的生长、发育和母体微生物之间的相互作用。为了研究地理或母体因素（分娩方式、母乳喂养方式、妊娠糖尿病）对新生儿肠道微生物群和噬菌体的影响，我们使用无菌容器收集了来自三个地区（恩施 9 对、呼和浩特 7 对、呼伦贝尔 18 对）的 34 对母婴分娩后 24 小时内的粪便样本。结果我们的研究结果表明，地理位置对母体肠道微生物有影响（P < 0.05），而对婴儿肠道微生物的影响不显著（P = 0.184）。在母体因素中，分娩方式对新生儿有显著影响（P < 0.05）。特定细菌（如乳酸杆菌、大肠埃希氏菌、大肠杆菌、人葡萄球菌、Veillonella spp.）、预测的活性代谢物和噬菌体 vOTU 随分娩方式的不同而变化。Phocaeicola vulgatus 与婴儿早期肠道中的某些代谢物和噬菌体有显著相关性（P &;lt;0.05）。总之，新生儿早期肠道微生物组（包括细菌和噬菌体）的定植受到分娩方式和母体妊娠糖尿病的严重影响。关键细菌可能与噬菌体相互作用，影响特定代谢物的水平。我们的研究为婴儿微生物组的研究提供了新的证据，填补了婴儿肠道微生物组病毒组分析的空白，并强调了母体健康对婴儿初始肠道病毒组的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cellular and Infection Microbiology IMMUNOLOGY-MICROBIOLOGY

CiteScore

7.90

自引率

7.00%

发文量

1817

审稿时长

14 weeks

期刊介绍： Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.

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