Hfq-binding small RNA PqsS regulates Pseudomonas aeruginosa pqs quorum sensing system and virulence

IF 7.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Tianyuan Jia, Xianbiao Bi, Menglu Li, Chenhui Zhang, Anmin Ren, Shangru Li, Tian Zhou, Yingdan Zhang, Yang Liu, Xue Liu, Yinyue Deng, Bin Liu, Guobao Li, Liang Yang
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引用次数: 0

Abstract

Pseudomonas aeruginosa is a widespread nosocomial pathogen with a significant to cause both severe planktonic acute and biofilm-related chronic infections. Small RNAs (sRNAs) are noncoding regulatory molecules that are stabilized by the RNA chaperone Hfq to trigger various virulence-related signaling pathways. Here, we identified an Hfq-binding sRNA in P. aeruginosa PAO1, PqsS, which promotes bacterial pathogenicity and pseudomonas quinolone signal quorum sensing (pqs QS) system. Specifically, PqsS enhanced acute bacterial infections by inducing host cell death and promoting rhamnolipid-regulated swarming motility. Meanwhile, PqsS reduced chronic infection traits including biofilm formation and antibiotic resistance. Moreover, PqsS repressed pqsL transcript, increasing PQS levels for pqs QS. A PQS-rich environment promoted PqsS expression, thus forming a positive feedback loop. Furthermore, we demonstrated that the PqsS interacts and destabilizes the pqsL mRNA by recruiting RNase E to drive degradation. These findings provide insights for future research on P. aeruginosa pathogenesis and targeted treatment.

Abstract Image

Hfq结合型小核糖核酸PqsS调控铜绿假单胞菌pqs法定人数感应系统和毒力
铜绿假单胞菌(Pseudomonas aeruginosa)是一种广泛存在的医院病原体,可引起严重的急性浮游生物感染和与生物膜相关的慢性感染。小 RNA(sRNA)是一种非编码调控分子,可通过 RNA 合子 Hfq 的稳定作用触发各种与毒力相关的信号通路。在这里,我们在铜绿假单胞菌 PAO1 中发现了一种与 Hfq 结合的 sRNA,即 PqsS,它能促进细菌的致病性和假单胞菌喹诺酮信号法定人数感应(pqs QS)系统。具体来说,PqsS 通过诱导宿主细胞死亡和促进鼠李糖脂调控的蜂拥运动,增强了急性细菌感染。同时,PqsS 减少了慢性感染特征,包括生物膜的形成和抗生素耐药性。此外,PqsS抑制了pqsL转录本,增加了pqs QS的PQS水平。富含 PQS 的环境促进了 PqsS 的表达,从而形成了一个正反馈回路。此外,我们还证明了 PqsS 通过招募 RNase E 来驱动降解,从而与 pqsL mRNA 相互作用并破坏其稳定性。这些发现为今后研究铜绿假单胞菌的致病机理和靶向治疗提供了启示。
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来源期刊
npj Biofilms and Microbiomes
npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology
CiteScore
12.10
自引率
3.30%
发文量
91
审稿时长
9 weeks
期刊介绍: npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.
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