Super-Resolution Microscopic Imaging of Lipid Droplets in Living Cells via Carbonized Polymer Dot-Based Polarity-Responsive Nanoprobe

IF 4.6 Q1 CHEMISTRY, ANALYTICAL

ACS Measurement Science Au Pub Date : 2024-09-06 DOI:10.1021/acsmeasuresciau.4c00049

Zepeng Huo, Zitong Yu, Weiqing Xu, Shuping Xu

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Abstract

Lipid droplets (LDs) are dynamic subcellular organelles that participate in various physiological processes, and their abnormality can also lead to various diseases. Tracing the dynamics of LDs in living cells will be valuable for understanding cell physiological states. Here, we employed a structured light illumination super-resolution imaging assisted with a carbonized polymer dot (CPD)-based fluorescence nanoprobe to track the travel paths of LDs and other organelles. The CPDs we developed are highly biocompatible with living cells and exhibit a highly sensitive response to solvent polarity, allowing for high specificity in staining LDs in living cells. Aided by these nanoprobes, we successfully observed many real-time LD-involved dynamics in living cells, such as intracellular LD interactions, communications with other organelles, and dynamic behaviors under external stimuli (oxidative stress inducer). These studies deepen our comprehension of the physiological role of LDs and drive the advancement of super-resolution fluorescent probes.

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通过基于碳化聚合物点的极性响应纳米探针对活细胞中的脂滴进行超分辨率显微成像

脂滴（LDs）是参与各种生理过程的动态亚细胞器，其异常也会导致各种疾病。追踪活细胞中脂滴的动态变化对了解细胞生理状态很有价值。在这里，我们采用了一种结构光照明超分辨率成像技术，辅助以碳化聚合物点（CPD）为基础的荧光纳米探针来跟踪 LDs 和其他细胞器的移动路径。我们开发的碳化聚合物点与活细胞具有高度的生物相容性，并对溶剂极性表现出高度敏感的反应，因此可对活细胞中的低密度脂蛋白进行高特异性染色。在这些纳米探针的帮助下，我们成功地观察到了活细胞中许多与 LD 有关的实时动态，如细胞内 LD 的相互作用、与其他细胞器的通讯以及在外部刺激（氧化应激诱导剂）下的动态行为。这些研究加深了我们对 LD 生理作用的理解，推动了超分辨率荧光探针的发展。

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来源期刊

ACS Measurement Science Au 化学计量学-

CiteScore

5.20

自引率

0.00%

发文量

期刊介绍： ACS Measurement Science Au is an open access journal that publishes experimental computational or theoretical research in all areas of chemical measurement science. Short letters comprehensive articles reviews and perspectives are welcome on topics that report on any phase of analytical operations including sampling measurement and data analysis. This includes:Chemical Reactions and SelectivityChemometrics and Data ProcessingElectrochemistryElemental and Molecular CharacterizationImagingInstrumentationMass SpectrometryMicroscale and Nanoscale systemsOmics (Genomics Proteomics Metabonomics Metabolomics and Bioinformatics)Sensors and Sensing (Biosensors Chemical Sensors Gas Sensors Intracellular Sensors Single-Molecule Sensors Cell Chips Arrays Microfluidic Devices)SeparationsSpectroscopySurface analysisPapers dealing with established methods need to offer a significantly improved original application of the method.