Injectable hybrid nanofibrous spheres made of PLA and nano-hydroxyapatite for cell delivery and osteogenic induction

IF 4.3 3区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Frontiers in Bioengineering and Biotechnology Pub Date : 2024-08-29 DOI:10.3389/fbioe.2024.1460870

Yawen Wang, Xiaopei Zhang, Na Liu, Renjie Chen, Chenghao Yu, Lijie Yao, Siyu Chen, Yuying Yan, Tong Wu, Yuanfei Wang

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引用次数: 0

Abstract

IntroductionNanofibrous spheres, with their injectable format and biomimetic three-dimensional topologies that emulate the complexity of natural extracellular environments, have become increasingly attractive for applications in biomedical and regenerative medicine. Our research contributes to this growing field by detailing the design and fabrication of a novel series of polylactic acid/nano-hydroxyapatite (PLA/nHA) hybrid nanofibrous spheres.MethodsThese advanced structures were created by integrating electrospinning and electrospray techniques, which allowed for precise control over the nanofibrous spheres, especially in size. We have conducted a comprehensive investigation into the nanofibrous spheres’ capacity to deliver stem cells efficiently and maintain their viability post-implantation, as well as their potential to induce osteogenic differentiation.Results and DiscussionThe results show that these nanofibrous spheres are biocompatible and injectable, effectively supporting the attachment, growth, and differentiation of bone marrow-derived mesenchymal stem cells while aiding in their targeted transportation to bone defect areas to execute their regenerative functions. The findings of this study could significantly impact the future development of biocompatible materials for a range of therapeutic applications, including bone tissue engineering and regenerative therapy.

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聚乳酸和纳米羟基磷灰石制成的可注射混合纳米纤维球用于细胞输送和成骨诱导

引言纳米纤维球具有可注射的形式和仿生物三维拓扑结构，可模拟复杂的天然细胞外环境，在生物医学和再生医学应用中的吸引力与日俱增。我们的研究通过详细介绍一系列新型聚乳酸/纳米羟基磷灰石（PLA/nHA）混合纳米纤维球的设计和制造，为这一不断发展的领域做出了贡献。结果与讨论结果表明，这些纳米纤维球具有生物相容性和可注射性，可有效支持骨髓间充质干细胞的附着、生长和分化，同时有助于将其定向运输到骨缺损区域，以发挥其再生功能。这项研究的发现可能会对未来用于一系列治疗应用的生物相容性材料的开发产生重大影响，包括骨组织工程和再生疗法。

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来源期刊

Frontiers in Bioengineering and Biotechnology Chemical Engineering-Bioengineering

CiteScore

8.30

自引率

5.30%

发文量

2270

审稿时长

12 weeks

期刊介绍： The translation of new discoveries in medicine to clinical routine has never been easy. During the second half of the last century, thanks to the progress in chemistry, biochemistry and pharmacology, we have seen the development and the application of a large number of drugs and devices aimed at the treatment of symptoms, blocking unwanted pathways and, in the case of infectious diseases, fighting the micro-organisms responsible. However, we are facing, today, a dramatic change in the therapeutic approach to pathologies and diseases. Indeed, the challenge of the present and the next decade is to fully restore the physiological status of the diseased organism and to completely regenerate tissue and organs when they are so seriously affected that treatments cannot be limited to the repression of symptoms or to the repair of damage. This is being made possible thanks to the major developments made in basic cell and molecular biology, including stem cell science, growth factor delivery, gene isolation and transfection, the advances in bioengineering and nanotechnology, including development of new biomaterials, biofabrication technologies and use of bioreactors, and the big improvements in diagnostic tools and imaging of cells, tissues and organs. In today`s world, an enhancement of communication between multidisciplinary experts, together with the promotion of joint projects and close collaborations among scientists, engineers, industry people, regulatory agencies and physicians are absolute requirements for the success of any attempt to develop and clinically apply a new biological therapy or an innovative device involving the collective use of biomaterials, cells and/or bioactive molecules. “Frontiers in Bioengineering and Biotechnology” aspires to be a forum for all people involved in the process by bridging the gap too often existing between a discovery in the basic sciences and its clinical application.