{"title":"DNA Barcoding and Intronic-ORF Structure Analyses of Cultivated Pyropia yezoensis in China: The Genetic Impact under Climate Change","authors":"Guihua Huang, Cuicui Tian, Liangdi Wei, Chuanming Hu, Guangping Xu, Wei Zhou, Yinyin Deng","doi":"10.3390/jmse12091551","DOIUrl":null,"url":null,"abstract":"Pyropia yezoensis is the most widely cultivated and economically important alga. Affected by climate change, the cultivation of P. yezoensis has gradually migrated to the northern coast of China, increasing from 6.8% in 2019 to 19.5% in 2023. To date, the genetic impact of northern migration on cultivation resources has not been assessed and analyzed extensively. Here, DNA barcoding (rbcL and cox1) and the presence/absence of intronic-ORFs in mitochondrial regions (rnl and cox1) were applied to investigate genetic diversity in 44 P. yezoensis specimens from 17 aquaculture farms in China, with comparisons to Korean and Japanese cultivated resources. The lower intraspecific variation was 0.31% for the cox1 gene and 0.14% for the rbcL gene, with three haplotypes, indicating that intensive selection and breeding during cultivation had narrowed the germplasm genetic variation. The intron structure of mitochondrial regions showed that the cultivated resources had 17 phenotypes, and the northern specimens shared 35.3% of genotypes with the southern specimens, indicating that the cultivated P. yezoensis is expanding its cultivation ranges through north migration. Even with lower genetic diversity, the northern area of cultivation had already developed 17.6% site-specific specimens. The genetic diversity of cultivated P. yezoensis from the Northwest Pacific is also discussed. Our work provides a preliminary framework for P. yezoensis breeding and cultivation under climate change.","PeriodicalId":16168,"journal":{"name":"Journal of Marine Science and Engineering","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Marine Science and Engineering","FirstCategoryId":"89","ListUrlMain":"https://doi.org/10.3390/jmse12091551","RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, MARINE","Score":null,"Total":0}
引用次数: 0
Abstract
Pyropia yezoensis is the most widely cultivated and economically important alga. Affected by climate change, the cultivation of P. yezoensis has gradually migrated to the northern coast of China, increasing from 6.8% in 2019 to 19.5% in 2023. To date, the genetic impact of northern migration on cultivation resources has not been assessed and analyzed extensively. Here, DNA barcoding (rbcL and cox1) and the presence/absence of intronic-ORFs in mitochondrial regions (rnl and cox1) were applied to investigate genetic diversity in 44 P. yezoensis specimens from 17 aquaculture farms in China, with comparisons to Korean and Japanese cultivated resources. The lower intraspecific variation was 0.31% for the cox1 gene and 0.14% for the rbcL gene, with three haplotypes, indicating that intensive selection and breeding during cultivation had narrowed the germplasm genetic variation. The intron structure of mitochondrial regions showed that the cultivated resources had 17 phenotypes, and the northern specimens shared 35.3% of genotypes with the southern specimens, indicating that the cultivated P. yezoensis is expanding its cultivation ranges through north migration. Even with lower genetic diversity, the northern area of cultivation had already developed 17.6% site-specific specimens. The genetic diversity of cultivated P. yezoensis from the Northwest Pacific is also discussed. Our work provides a preliminary framework for P. yezoensis breeding and cultivation under climate change.
期刊介绍:
Journal of Marine Science and Engineering (JMSE; ISSN 2077-1312) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to marine science and engineering. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.