DNA Barcoding and Intronic-ORF Structure Analyses of Cultivated Pyropia yezoensis in China: The Genetic Impact under Climate Change

IF 2.7 3区 地球科学 Q1 ENGINEERING, MARINE
Guihua Huang, Cuicui Tian, Liangdi Wei, Chuanming Hu, Guangping Xu, Wei Zhou, Yinyin Deng
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Abstract

Pyropia yezoensis is the most widely cultivated and economically important alga. Affected by climate change, the cultivation of P. yezoensis has gradually migrated to the northern coast of China, increasing from 6.8% in 2019 to 19.5% in 2023. To date, the genetic impact of northern migration on cultivation resources has not been assessed and analyzed extensively. Here, DNA barcoding (rbcL and cox1) and the presence/absence of intronic-ORFs in mitochondrial regions (rnl and cox1) were applied to investigate genetic diversity in 44 P. yezoensis specimens from 17 aquaculture farms in China, with comparisons to Korean and Japanese cultivated resources. The lower intraspecific variation was 0.31% for the cox1 gene and 0.14% for the rbcL gene, with three haplotypes, indicating that intensive selection and breeding during cultivation had narrowed the germplasm genetic variation. The intron structure of mitochondrial regions showed that the cultivated resources had 17 phenotypes, and the northern specimens shared 35.3% of genotypes with the southern specimens, indicating that the cultivated P. yezoensis is expanding its cultivation ranges through north migration. Even with lower genetic diversity, the northern area of cultivation had already developed 17.6% site-specific specimens. The genetic diversity of cultivated P. yezoensis from the Northwest Pacific is also discussed. Our work provides a preliminary framework for P. yezoensis breeding and cultivation under climate change.
中国栽培罂粟的DNA条形码和Intronic-ORF结构分析:气候变化对遗传的影响
酵母藻(Pyropia yezoensis)是栽培面积最大、经济价值最高的藻类。受气候变化的影响,酵母藻的栽培已逐渐向中国北部沿海迁移,从 2019 年的 6.8% 增加到 2023 年的 19.5%。迄今为止,北移对栽培资源的遗传影响尚未得到广泛评估和分析。在此,应用DNA条形码(rbcL和cox1)和线粒体区(rnl和cox1)内含子-ORFs的存在/缺失研究了来自中国17个水产养殖场的44个叶氏鲈标本的遗传多样性,并与韩国和日本的养殖资源进行了比较。结果表明,cox1 基因的种内变异较低,为 0.31%;rbcL 基因的种内变异较低,为 0.14%,有 3 个单倍型,表明养殖过程中的强化选育缩小了种质遗传变异。线粒体区的内含子结构显示,栽培资源有17个表型,北方标本与南方标本共享35.3%的基因型,表明栽培的酵母属植物正通过北移扩大其栽培范围。即使在遗传多样性较低的情况下,北方栽培区也已经形成了 17.6%的地点特异性标本。我们还讨论了西北太平洋栽培酵母的遗传多样性。我们的工作为气候变化条件下叶茜草的育种和栽培提供了一个初步框架。
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来源期刊
Journal of Marine Science and Engineering
Journal of Marine Science and Engineering Engineering-Ocean Engineering
CiteScore
4.40
自引率
20.70%
发文量
1640
审稿时长
18.09 days
期刊介绍: Journal of Marine Science and Engineering (JMSE; ISSN 2077-1312) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to marine science and engineering. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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