Passive Immunotherapy of Cynomolgus Monkeys with Anti-Rotavirus IgY

IF 4.9 3区医学 Q1 PHARMACOLOGY & PHARMACY

Pharmaceutics Pub Date : 2024-08-30 DOI:10.3390/pharmaceutics16091149

Gentil Arthur Bentes, Juliana Rodrigues Guimarães, Eduardo de Mello Volotão, Natália Maria Lanzarini, Alexandre dos Santos da Silva, Noemi Rovaris Gardinali, Renato Sergio Marchevsky, José Paulo Gagliardi Leite, Jaqueline Mendes de Oliveira, Marcelo Alves Pinto

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引用次数: 0

Abstract

Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey (Macaca fascicularis) model. Animals were experimentally infected with human rotavirus Group A (RVA), the most common cause of severe acute diarrhoea among young children worldwide. Animals were administered human RVA (3.1 × 107 FFU/mL) by oral gavage, challenged with 2.5 mg of anti-RVA IgY orally, and monitored for five days according to clinical, haematological and biochemical parameters; serum electrolyte levels; viral shedding; and histopathological changes. Immunotherapy with anti-RVA IgY had a protective effect against severe rotavirus-induced enteritis in four of the ten treated monkeys, as evidenced by histopathological findings. Although only one animal had diarrhoea, all but one exhibited virus shedding regardless of the treatment.

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用抗轮状病毒 IgY 对猕猴进行被动免疫治疗

从母鸡蛋黄中提纯的免疫球蛋白 Y（IgY）是开发新型诊断和治疗平台的一种极具吸引力和成本效益的替代品。在这项研究中，我们评估了轮状病毒特异性 IgY 在猕猴（Macaca fascicularis）模型中的治疗效果。实验动物感染了人类 A 组轮状病毒（RVA），这是全球幼儿严重急性腹泻最常见的病因。通过口服灌胃给动物注射人类 RVA（3.1 × 107 FFU/mL），口服 2.5 毫克抗 RVA IgY，并根据临床、血液学和生化参数、血清电解质水平、病毒脱落和组织病理学变化对动物进行为期五天的监测。组织病理学结果表明，10 只接受抗 RVA IgY 免疫疗法的猴子中有 4 只对轮状病毒引起的严重肠炎具有保护作用。虽然只有一只猴子出现腹泻，但无论治疗与否，除一只猴子外，其他所有猴子都出现了病毒脱落。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutics Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

7.90

自引率

11.10%

发文量

2379

审稿时长

16.41 days

期刊介绍： Pharmaceutics (ISSN 1999-4923) is an open access journal which provides an advanced forum for the science and technology of pharmaceutics and biopharmaceutics. It publishes reviews, regular research papers, communications, and short notes. Covered topics include pharmacokinetics, toxicokinetics, pharmacodynamics, pharmacogenetics and pharmacogenomics, and pharmaceutical formulation. Our aim is to encourage scientists to publish their experimental and theoretical details in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.