Protection against DSS-induced colitis in mice through FcεRIα deficiency: the role of altered Lactobacillus

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-09-12 DOI:10.1038/s41522-024-00563-z

Yue Yin, Ruilong Wang, Yanning Li, Wenfei Qin, Letian Pan, Chenyuan Yan, Yusen Hu, Guangqiang Wang, Lianzhong Ai, Qixiang Mei, Li Li

{"title":"Protection against DSS-induced colitis in mice through FcεRIα deficiency: the role of altered Lactobacillus","authors":"Yue Yin, Ruilong Wang, Yanning Li, Wenfei Qin, Letian Pan, Chenyuan Yan, Yusen Hu, Guangqiang Wang, Lianzhong Ai, Qixiang Mei, Li Li","doi":"10.1038/s41522-024-00563-z","DOIUrl":null,"url":null,"abstract":"The role of mast cells (MCs) in ulcerative colitis (UC) development is controversial. FcεRI, the IgE high-affinity receptor, is known to activate MCs. However, its role in UC remains unclear. In our study, Anti-FcεRI showed highly diagnostic value for UC. FcεRIα knockout in mice ameliorated DSS-induced colitis in a gut microbiota-dependent manner. Increased Lactobacillus abundance in FcεRIα deficient mice showed strongly correlation with the remission of colitis. RNA sequencing indicated activation of the NLRP6 inflammasome pathway in FcεRIα knockout mice. Additionally, Lactobacillus plantarum supplementation protected against inflammatory injury and goblet cell loss, with activation of the NLRP6 inflammasome during colitis. Notably, this effect was absent when the strain is unable to produce lactic acid. In summary, colitis was mitigated in FcεRIα deficient mice, which may be attributed to the increased abundance of Lactobacillus. These findings contribute to a better understanding of the relationship between allergic reactions, microbiota, and colitis.","PeriodicalId":19370,"journal":{"name":"npj Biofilms and Microbiomes","volume":null,"pages":null},"PeriodicalIF":7.8000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"npj Biofilms and Microbiomes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41522-024-00563-z","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The role of mast cells (MCs) in ulcerative colitis (UC) development is controversial. FcεRI, the IgE high-affinity receptor, is known to activate MCs. However, its role in UC remains unclear. In our study, Anti-FcεRI showed highly diagnostic value for UC. FcεRIα knockout in mice ameliorated DSS-induced colitis in a gut microbiota-dependent manner. Increased Lactobacillus abundance in FcεRIα deficient mice showed strongly correlation with the remission of colitis. RNA sequencing indicated activation of the NLRP6 inflammasome pathway in FcεRIα knockout mice. Additionally, Lactobacillus plantarum supplementation protected against inflammatory injury and goblet cell loss, with activation of the NLRP6 inflammasome during colitis. Notably, this effect was absent when the strain is unable to produce lactic acid. In summary, colitis was mitigated in FcεRIα deficient mice, which may be attributed to the increased abundance of Lactobacillus. These findings contribute to a better understanding of the relationship between allergic reactions, microbiota, and colitis.

Abstract Image

查看原文本刊更多论文

通过 FcεRIα 缺乏保护小鼠免受 DSS 引起的结肠炎：乳酸杆菌改变的作用

肥大细胞（MCs）在溃疡性结肠炎（UC）发病中的作用尚存争议。已知 IgE 高亲和力受体 FcεRI 可激活肥大细胞。然而，它在 UC 中的作用仍不清楚。在我们的研究中，抗 FcεRI 对 UC 有很高的诊断价值。小鼠FcεRIα基因敲除能以肠道微生物群依赖的方式改善DSS诱导的结肠炎。FcεRIα缺陷小鼠体内乳酸杆菌数量的增加与结肠炎的缓解密切相关。RNA 测序表明，FcεRIα基因敲除小鼠的 NLRP6 炎性体通路被激活。此外，在结肠炎期间，补充植物乳杆菌可保护NLRP6炎症体活化，防止炎症损伤和鹅口疮细胞损失。值得注意的是，当菌株不能产生乳酸时，就不会产生这种效果。总之，FcεRIα缺陷小鼠的结肠炎得到缓解，这可能归因于乳酸杆菌数量的增加。这些发现有助于更好地理解过敏反应、微生物群和结肠炎之间的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.

文献相关原料

公司名称	产品信息	采购帮参考价格