Neima K. Al-Senosy, Noura El-Kattan, Enas A. Hassan, Hemmat M. Abd-Elhady, Abdelkader Hazem, Mona A. Ashour, Mosaad A. Abdel-Wahhab
{"title":"Therapeutic Role of Secondary Metabolites from Probiotic Strains for Ehrlich Solid Tumors in Mice","authors":"Neima K. Al-Senosy, Noura El-Kattan, Enas A. Hassan, Hemmat M. Abd-Elhady, Abdelkader Hazem, Mona A. Ashour, Mosaad A. Abdel-Wahhab","doi":"10.1007/s00284-024-03864-w","DOIUrl":null,"url":null,"abstract":"<p>This study aimed to screen the bioactive components in <i>Streptococcus equinus</i> WC<sub>1</sub> (SE-WC<sub>1</sub>) and <i>Limosilactobacillus reuteri</i> GM<sub>4</sub> (LR-GM<sub>4</sub>) and estimate the therapeutic role in Ehrlich solid tumors (EST) mice model. Forty-four male albino EST mice were assigned into 7 groups and treated daily for 2 weeks, including the EST group, the EST mice that received SE-WC<sub>1</sub> at a low or a high dose (0.5 ml *10<sup>6</sup> or 0.5 ml *10<sup>8</sup> cfu), the EST mice that received LR-GM<sub>4</sub> at the low or the high dose (0.5 ml *10<sup>6</sup> or 0.5 ml *10<sup>8</sup> cfu), and the EST mice that received SE-WC<sub>1</sub> plus LR-GM<sub>4</sub> at the low or the high dose. Tumors were harvested, weighed, examined, and used for the determination of apoptosis-related gene expression. Samples of the intestine, liver, and kidney were gathered for histological examination. The GC–MS identified 24 and 36 bioactive compounds in SE-WC<sub>1</sub> and LR-GM<sub>4</sub>, respectively. The main compound in SE-WC<sub>1</sub> was lupeol; however, the main compound in LR-GM<sub>4</sub> was retinaldehyde. EST mice showed disturbances in Bcl-2, Bax, and p53 mRNA expression along with histological changes in the intestine, liver, and kidney. Administration of both bacterial strains reduced the tumor weight, alleviated the disturbances in the gene expression, and improved the histological structure of the intestine, liver, and kidney in a dose-dependent. Moreover, LR-GM<sub>4</sub> was more effective than SE-WC<sub>1</sub> due to its higher content of bioactive compounds. It could be concluded that these strains of probiotics are promising for the treatment of solid tumors.</p>","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"13 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-03864-w","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to screen the bioactive components in Streptococcus equinus WC1 (SE-WC1) and Limosilactobacillus reuteri GM4 (LR-GM4) and estimate the therapeutic role in Ehrlich solid tumors (EST) mice model. Forty-four male albino EST mice were assigned into 7 groups and treated daily for 2 weeks, including the EST group, the EST mice that received SE-WC1 at a low or a high dose (0.5 ml *106 or 0.5 ml *108 cfu), the EST mice that received LR-GM4 at the low or the high dose (0.5 ml *106 or 0.5 ml *108 cfu), and the EST mice that received SE-WC1 plus LR-GM4 at the low or the high dose. Tumors were harvested, weighed, examined, and used for the determination of apoptosis-related gene expression. Samples of the intestine, liver, and kidney were gathered for histological examination. The GC–MS identified 24 and 36 bioactive compounds in SE-WC1 and LR-GM4, respectively. The main compound in SE-WC1 was lupeol; however, the main compound in LR-GM4 was retinaldehyde. EST mice showed disturbances in Bcl-2, Bax, and p53 mRNA expression along with histological changes in the intestine, liver, and kidney. Administration of both bacterial strains reduced the tumor weight, alleviated the disturbances in the gene expression, and improved the histological structure of the intestine, liver, and kidney in a dose-dependent. Moreover, LR-GM4 was more effective than SE-WC1 due to its higher content of bioactive compounds. It could be concluded that these strains of probiotics are promising for the treatment of solid tumors.
期刊介绍:
Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment.
Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas:
physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.