A Simple and Efficient Colorimetric Detection of Creatinine Based on Citrate-Stabilized Gold Nanoparticles

IF 3.3 4区 物理与天体物理 Q2 CHEMISTRY, PHYSICAL
Xianfa Lv, Tongrui Shi, Xia Bai, Zheng Guan, Huanran Wang, Rujian Jiang, Lu Zhou, Hongyu Chen
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Abstract

Creatinine level is a crucial indicator in the clinical assessment and diagnosis of renal diseases, and achieving simple and accurate detection of urinary creatinine levels in resource-limited point-of-care settings is of great significance in the timely prevention and diagnosis of kidney diseases. As a popular zero-dimensional material, gold nanoparticles (AuNPs) exhibit intriguing optical properties and thus have become a promising material for many sensing detection applications. Here, we proposed a simple, efficient, and sensitive quantitative detection of creatinine by studying the relative absorbance (ΔA) of AuNPs in absence and presence of creatinine. The method relies on the aggregation of AuNPs via ligand-exchanged of citrate ions and creatinine on the surface of AuNPs to achieve colorimetric detection. With this assay, the limit of detection for creatinine was as low as 0.16 mM, and the dynamic detection range was 0.5 to 20 mM under optimized conditions. In our experiments, the specificity of the proposed method was investigated and successfully applied to detect creatinine in urine sample. It reveals that the proposed colorimetric protocol has demonstrated a high sensitivity and selectivity for creatinine, and has a potential practicability in clinical diagnostics.

Abstract Image

基于柠檬酸盐稳定金纳米粒子的简单高效比色法检测肌酐
肌酐水平是临床评估和诊断肾脏疾病的重要指标,在资源有限的医疗点实现简单而准确的尿肌酐水平检测,对于及时预防和诊断肾脏疾病具有重要意义。作为一种常用的零维材料,金纳米粒子(AuNPs)表现出引人入胜的光学特性,因此已成为许多传感检测应用领域的一种前景广阔的材料。在此,我们提出了一种简单、高效、灵敏的肌酐定量检测方法,即研究 AuNPs 在无肌酐和有肌酐时的相对吸光度 (ΔA)。该方法通过柠檬酸根离子和肌酐在 AuNPs 表面的配体交换使 AuNPs 聚合,从而实现比色检测。在优化条件下,肌酐的检测限低至 0.16 mM,动态检测范围为 0.5 至 20 mM。我们在实验中考察了所提方法的特异性,并成功地将其用于检测尿样中的肌酐。结果表明,所提出的比色方案对肌酐具有较高的灵敏度和选择性,在临床诊断中具有潜在的实用性。
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来源期刊
Plasmonics
Plasmonics 工程技术-材料科学:综合
CiteScore
5.90
自引率
6.70%
发文量
164
审稿时长
2.1 months
期刊介绍: Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons. Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.
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