{"title":"A Simple and Efficient Colorimetric Detection of Creatinine Based on Citrate-Stabilized Gold Nanoparticles","authors":"Xianfa Lv, Tongrui Shi, Xia Bai, Zheng Guan, Huanran Wang, Rujian Jiang, Lu Zhou, Hongyu Chen","doi":"10.1007/s11468-024-02510-2","DOIUrl":null,"url":null,"abstract":"<p>Creatinine level is a crucial indicator in the clinical assessment and diagnosis of renal diseases, and achieving simple and accurate detection of urinary creatinine levels in resource-limited point-of-care settings is of great significance in the timely prevention and diagnosis of kidney diseases. As a popular zero-dimensional material, gold nanoparticles (AuNPs) exhibit intriguing optical properties and thus have become a promising material for many sensing detection applications. Here, we proposed a simple, efficient, and sensitive quantitative detection of creatinine by studying the relative absorbance (Δ<i>A</i>) of AuNPs in absence and presence of creatinine. The method relies on the aggregation of AuNPs via ligand-exchanged of citrate ions and creatinine on the surface of AuNPs to achieve colorimetric detection. With this assay, the limit of detection for creatinine was as low as 0.16 mM, and the dynamic detection range was 0.5 to 20 mM under optimized conditions. In our experiments, the specificity of the proposed method was investigated and successfully applied to detect creatinine in urine sample. It reveals that the proposed colorimetric protocol has demonstrated a high sensitivity and selectivity for creatinine, and has a potential practicability in clinical diagnostics.</p>","PeriodicalId":736,"journal":{"name":"Plasmonics","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plasmonics","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1007/s11468-024-02510-2","RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Creatinine level is a crucial indicator in the clinical assessment and diagnosis of renal diseases, and achieving simple and accurate detection of urinary creatinine levels in resource-limited point-of-care settings is of great significance in the timely prevention and diagnosis of kidney diseases. As a popular zero-dimensional material, gold nanoparticles (AuNPs) exhibit intriguing optical properties and thus have become a promising material for many sensing detection applications. Here, we proposed a simple, efficient, and sensitive quantitative detection of creatinine by studying the relative absorbance (ΔA) of AuNPs in absence and presence of creatinine. The method relies on the aggregation of AuNPs via ligand-exchanged of citrate ions and creatinine on the surface of AuNPs to achieve colorimetric detection. With this assay, the limit of detection for creatinine was as low as 0.16 mM, and the dynamic detection range was 0.5 to 20 mM under optimized conditions. In our experiments, the specificity of the proposed method was investigated and successfully applied to detect creatinine in urine sample. It reveals that the proposed colorimetric protocol has demonstrated a high sensitivity and selectivity for creatinine, and has a potential practicability in clinical diagnostics.
期刊介绍:
Plasmonics is an international forum for the publication of peer-reviewed leading-edge original articles that both advance and report our knowledge base and practice of the interactions of free-metal electrons, Plasmons.
Topics covered include notable advances in the theory, Physics, and applications of surface plasmons in metals, to the rapidly emerging areas of nanotechnology, biophotonics, sensing, biochemistry and medicine. Topics, including the theory, synthesis and optical properties of noble metal nanostructures, patterned surfaces or materials, continuous or grated surfaces, devices, or wires for their multifarious applications are particularly welcome. Typical applications might include but are not limited to, surface enhanced spectroscopic properties, such as Raman scattering or fluorescence, as well developments in techniques such as surface plasmon resonance and near-field scanning optical microscopy.