Scalable Ammonia Synthesis in Fermentors Using Quantum Dot-Azotobacter vinelandii Hybrids

IF 2.9 4区 工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY
Jayeong Kim, Byunghyun Lee, Gui-Min Kim, Ilsong Lee, Sang Yup Lee, Kyeong Rok Choi, Doh C. Lee
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Abstract

This study introduces a scalable synthesis of ammonia through photochemical reactions, wherein nitrogen-fixing bacterial cells, Azotobacter vinelandii (A. vinelandii), form hybrids with colloidal quantum dots (QDs). Irradiation of the QD-A. vinelandii hybrids with visible light is found to significantly enhance ammonia production efficiency. The inherently low ammonia conversion rate of wild-type A. vinelandii is substantially increased upon incorporation of QDs. This increase is attributed to the electron transfer from QDs within the bacterial cells to intracellular bio-components. Transferring this chemistry to a large-scale reaction presents a tremendous challenge, as it requires precise control over the growth conditions. We explore the scalability of the QD-A. vinelandii hybrids by conducting the photochemical reaction in a 5-L fermentor under various parameters, such as dissolved oxygen, nutrient supply, and pH. Interestingly, ammonia was produced in media depleted of carbon sources. Consequently, a two-step fermentation process was designed, enabling effective ammonia production. Our findings demonstrate that the QD-A. vinelandii hybrid system in a bioreactor setup achieves an ammonia turnover frequency of 11.96 s−1, marking a more than sixfold increase in efficiency over that of nitrogenase enzymes alone. This advancement highlights the potential of integrating biological and nanotechnological elements for scalable ammonia production processes.

Abstract Image

利用量子点-醋兰氮杂菌在发酵罐中进行可扩展的氨合成
本研究介绍了一种通过光化学反应合成氨的可扩展方法,其中固氮细菌细胞--醋蓝绿藻(Azotobacter vinelandii,A. vinelandii)与胶体量子点(QDs)形成杂交体。研究发现,用可见光照射 QD-A. vinelandii 杂交细胞可显著提高氨生产效率。加入 QDs 后,野生型葡萄酵母固有的低氨转化率大幅提高。这种提高归因于细菌细胞内 QDs 向细胞内生物成分的电子转移。将这种化学反应转化为大规模反应是一项巨大的挑战,因为它需要对生长条件进行精确控制。我们探索了 QD-A. vinelandii 杂交菌的可扩展性,方法是在一个 5 升的发酵罐中,在不同参数(如溶解氧、营养供应和 pH 值)下进行光化学反应。有趣的是,在缺乏碳源的培养基中会产生氨。因此,我们设计了一个两步发酵过程,从而实现了有效的氨生产。我们的研究结果表明,在生物反应器设置中,QD-A. vinelandii 混合系统的氨周转频率达到 11.96 s-1,比单独使用氮酶的效率提高了六倍多。这一进步凸显了将生物和纳米技术元素整合到可扩展的氨生产过程中的潜力。
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来源期刊
Korean Journal of Chemical Engineering
Korean Journal of Chemical Engineering 工程技术-工程:化工
CiteScore
4.60
自引率
11.10%
发文量
310
审稿时长
4.7 months
期刊介绍: The Korean Journal of Chemical Engineering provides a global forum for the dissemination of research in chemical engineering. The Journal publishes significant research results obtained in the Asia-Pacific region, and simultaneously introduces recent technical progress made in other areas of the world to this region. Submitted research papers must be of potential industrial significance and specifically concerned with chemical engineering. The editors will give preference to papers having a clearly stated practical scope and applicability in the areas of chemical engineering, and to those where new theoretical concepts are supported by new experimental details. The Journal also regularly publishes featured reviews on emerging and industrially important subjects of chemical engineering as well as selected papers presented at international conferences on the subjects.
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