Signal-amplified detection of amyloid-β aggregates based on plasmon-enhanced fluorescence

IF 4.9 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchemical Journal Pub Date : 2024-08-31 DOI:10.1016/j.microc.2024.111541

Wen Sun, Cheng Li, Jingtian Yu, Xiaoyan Liang, Xiaochen Gao, Baoxing Shen, Yaqiong Yang

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引用次数: 0

Abstract

Protein aggregation and misfolding are the main causes of neurodegenerative diseases. The development of a sensitive detection method and early diagnosis is significant for the prevention, diagnosis and treatment of these diseases. Herein, boron dipyrromethene (BODIPY)-modified probe based on plasmon-enhanced fluorescence (PEF) was developed for the detection of amyloid-β protein (Aβ) aggregates. Gold nanoparticles (Au NPs) and BODIPY were as the plasma, fluorescent substance, respectively. Meanwhile, the distance between the Au NPs and the BODIPY was controlled by silica spacer. Compared with the original BODIPY, the fluorescence signal of BODIPY-decorated Au NPs (Au NPs@SiO-BODIPY) exhibited 13-fold fluorescence enhancement. Three dimensions finite difference time domain (3D-FDTD) result demonstrated the signal-amplified was ascribed to the increase of electric field intensity (3.78 V/m). The linear range and detection limit for Aβ detection were lowered to 5–60 μM and 0.85 μM. The developed PEF probe was applied to selective and sensitive recognize Aβ aggregates in mouse colorectal carcinoma cells (CT26) and mouse pancreatic cancer cells (PAN02), which also showed a higher fluorescent signal than the original BODIPY. This method has a broad application prospect in the prediction and detection of neurodegenerative diseases.

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基于等离子体增强荧光的淀粉样蛋白-β聚集体信号增强检测

蛋白质聚集和错误折叠是导致神经退行性疾病的主要原因。开发灵敏的检测方法和早期诊断对这些疾病的预防、诊断和治疗具有重要意义。在此，我们开发了基于等离子体增强荧光（PEF）的硼二吡咯烷酮（BODIPY）修饰探针，用于检测淀粉样β蛋白（Aβ）聚集。金纳米粒子（Au NPs）和 BODIPY 分别作为血浆和荧光物质。同时，Au NPs 与 BODIPY 之间的距离由二氧化硅间隔控制。与原始 BODIPY 相比，BODIPY 装饰的金纳米粒子（Au NPs@SiO-BODIPY）的荧光信号增强了 13 倍。三维有限差分时域（3D-FDTD）结果表明，信号增强归因于电场强度（3.78 V/m）的增加。Aβ 检测的线性范围和检测限分别降至 5-60 μM 和 0.85 μM。所开发的 PEF 探针被应用于选择性地、灵敏地识别小鼠结直肠癌细胞（CT26）和小鼠胰腺癌细胞（PAN02）中的 Aβ 聚集体，其荧光信号也高于原来的 BODIPY。该方法在预测和检测神经退行性疾病方面具有广阔的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchemical Journal 化学-分析化学

CiteScore

8.70

自引率

8.30%

发文量

1131

审稿时长

1.9 months

期刊介绍： The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field. Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.