{"title":"Product catalysis dual entropy-driven amplification reaction strategy for miRNA-21 detection in glioblastoma","authors":"Linbo Zou, Xiaojun Liu, Lizhu Yang, Wen Yun","doi":"10.1016/j.microc.2024.111515","DOIUrl":null,"url":null,"abstract":"MicroRNA is taken as diagnostic tumor markers in clinical diagnosis of various cancers. However, the complexity system and low utilization rate of entropy-driven amplification reaction (EDAR) limit its application. In this work, a product catalysis dual EDAR amplification strategy is developed for miRNA-21 detection. This strategy allows miRNA-21 to simultaneously catalyze two EDARs, greatly increasing the limit of detection to 0.40 pM and enhancing amplification efficiency. The amplification efficiency was also significantly enhanced with a much shorter reaction time by increasing concentration of the catalyzer (EDAR product). The approach was successfully applied in clinical samples, showing potential for early screening and diagnosis.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.9000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchemical Journal","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.microc.2024.111515","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
MicroRNA is taken as diagnostic tumor markers in clinical diagnosis of various cancers. However, the complexity system and low utilization rate of entropy-driven amplification reaction (EDAR) limit its application. In this work, a product catalysis dual EDAR amplification strategy is developed for miRNA-21 detection. This strategy allows miRNA-21 to simultaneously catalyze two EDARs, greatly increasing the limit of detection to 0.40 pM and enhancing amplification efficiency. The amplification efficiency was also significantly enhanced with a much shorter reaction time by increasing concentration of the catalyzer (EDAR product). The approach was successfully applied in clinical samples, showing potential for early screening and diagnosis.
期刊介绍:
The Microchemical Journal is a peer reviewed journal devoted to all aspects and phases of analytical chemistry and chemical analysis. The Microchemical Journal publishes articles which are at the forefront of modern analytical chemistry and cover innovations in the techniques to the finest possible limits. This includes fundamental aspects, instrumentation, new developments, innovative and novel methods and applications including environmental and clinical field.
Traditional classical analytical methods such as spectrophotometry and titrimetry as well as established instrumentation methods such as flame and graphite furnace atomic absorption spectrometry, gas chromatography, and modified glassy or carbon electrode electrochemical methods will be considered, provided they show significant improvements and novelty compared to the established methods.