Immobilization of Arginase Using the Organic-Inorganic Hybrid Nanoflower Strategy for L-Ornithine Production

Abstract

The utilization of arginase for catalyzing the synthesis of L-arginine into L-ornithine has proven to be an effective industrial production process. The poor stability of arginase hampers its synthesis efficiency. Organic-inorganic hybrid nanoflowers serve as an effective strategy for enzyme immobilization. However, the precipitation of such immobilized enzymes is prone to dissolution and destruction in amino acid solutions, limiting their applicability. This paper systematically investigates the solubility of organic-inorganic hybrid nanoflowers formed with common metal ions in typical amino acid solutions. Additionally, a novel approach involving the preparation of calcium phosphate hybrid nanoflowers using crude arginase is developed. The findings confirm that the immobilized arginase exhibits superior stability and a broader application range. In the reaction system where L-arginine is utilized as a substrate for synthesizing ornithine, the immobilized arginase demonstrates higher substrate conversion rates and ornithine concentrations compared to free arginase crude extract. This approach holds the potential for industrial applications due to its improved performance.