Rapid detection of OTA and ZEN with dual quantum dots fluorescence immunochromatographic test strip

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-08-13 DOI:10.1007/s12161-024-02662-1

Qingbao Yang, Huawen Yan, Yumei Chen, Enping Liu, Chao Liang, Jingming Zhou, Aiping Wang

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Abstract

Mycotoxin contamination in agri-food systems has been a serious global concern over the past few decades. Corn is easily contaminated by Ochratoxin A (OTA) and Zearalenone (ZEN), which seriously threaten the survival and health of humans and animals. Herein, a rapid and sensitive method of OTA and ZEN dual quantum dots (QDs) fluorescence immunochromatographic test strip was established in this study. OTA and ZEN fluorescence probes were prepared by carbodiimide. The OTA-OVA, ZEN-BSA and staphylococcal protein A were sprayed on the nitrocellulose filter membrane as the T1 line, T2 line and control line of the dual test strip, which exhibited high sensitivity, accuracy and specificity. For quantitative detection of OTA, the linear regression equation was y = 0.3222x + 0.3834 (R² = 0.9687), LOD and IC₅₀ were 0.132 ng/mL and 2.296 ng/mL, and the linear detection range was 0.269 ng/mL to 19.588 ng/mL. In addition, for quantitative detection of ZEN, its linear regression equation was y = 0.3077x + 0.3777 (R² = 0.9648), LOD and IC₅₀ were 0.125 ng/mL and 2.495 ng/mL, and the linear detection range was 0.264 ng/mL to 23.55 ng/mL. Briefly, the OTA and ZEN dual QDs immunochromatographic test strip was favored for the simultaneous detection of OTA and ZEN in corn.

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利用双量子点荧光免疫层析试纸条快速检测 OTA 和 ZEN

过去几十年来，农业食品系统中的霉菌毒素污染一直是全球严重关切的问题。玉米很容易受到赭曲霉毒素 A（OTA）和玉米赤霉烯酮（ZEN）的污染，严重威胁人类和动物的生存与健康。本研究建立了一种快速灵敏的 OTA 和 ZEN 双量子点（QDs）荧光免疫层析检测试纸条。OTA 和 ZEN 荧光探针由碳二亚胺制备。将 OTA-OVA、ZEN-BSA 和葡萄球菌蛋白 A 分别喷涂在硝酸纤维素滤膜上，分别作为双试纸的 T1 线、T2 线和对照线，具有较高的灵敏度、准确性和特异性。在定量检测 OTA 时，线性回归方程为 y = 0.3222x + 0.3834（R2 = 0.9687），LOD 和 IC50 分别为 0.132 ng/mL 和 2.296 ng/mL，线性检测范围为 0.269 ng/mL 至 19.588 ng/mL。此外，在定量检测 ZEN 时，其线性回归方程为 y = 0.3077x + 0.3777（R2 = 0.9648），LOD 和 IC50 分别为 0.125 ng/mL 和 2.495 ng/mL，线性检测范围为 0.264 ng/mL 至 23.55 ng/mL。简而言之，该 OTA 和 ZEN 双 QDs 免疫层析试纸条可用于同时检测玉米中的 OTA 和 ZEN。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.