A pro-healing short-chain antimicrobial peptide that inhibits sepsis

IF 6.8 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Science China Materials Pub Date : 2024-08-28 DOI:10.1007/s40843-024-3002-0

Zekai Ren (, ), Xin Ding (, ), Yumei Wang (, ), Han Wu (, ), Xin Liu (, ), Yang Cao (, ), Hailin Cong (, ), Youqing Shen (, ), Bing Yu (, )

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Abstract

Chronic wounds experiencing infections with multidrug-resistant bacteria can be fatal, and in severe cases can lead to sepsis. Antimicrobial peptides are widely used in the field of wound care for their broad-spectrum antibacterial properties and good anti-drug resistance. We prepared bacterial cell membrane chromatography (BCMC) by extracting cell membranes of bacteria using SiO₂ microspheres as stationary phase. A library of antimicrobial peptides was synthesized in solid phase and screened by BCMC to identify the antimicrobial peptide LKAHR (later named LS5), which is characterized by biosafety, broad-spectrum antibacterial activity, and drug resistance, and a gelatin-based antimicrobial hydrogel (LS5-gel) was prepared to be better applied to wounds. LS5-gel was found to have good in vivo bactericidal properties as well as the ability to promote wound healing in a wound healing model. In the sepsis model, LS5 was found to have a significant inhibitory effect on sepsis infection. It is important for the selection of next-generation antimicrobial drugs and the treatment of chronic wound healing.

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一种能抑制败血症的促愈合短链抗菌肽

慢性伤口感染耐多药细菌可致命，严重时可导致败血症。抗菌肽具有广谱抗菌特性和良好的抗药性，被广泛应用于伤口护理领域。我们以二氧化硅微球为固定相，通过提取细菌细胞膜制备了细菌细胞膜色谱（BCMC）。在固相中合成了抗菌肽库，并通过 BCMC 进行筛选，确定了具有生物安全性、广谱抗菌活性和耐药性的抗菌肽 LKAHR（后命名为 LS5），并制备了明胶基抗菌水凝胶（LS5-凝胶），以便更好地应用于伤口。研究发现，LS5-凝胶具有良好的体内杀菌特性，并能在伤口愈合模型中促进伤口愈合。在败血症模型中，LS5 对败血症感染有明显的抑制作用。这对选择下一代抗菌药物和治疗慢性伤口愈合具有重要意义。

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来源期刊

Science China Materials Materials Science-General Materials Science

CiteScore

11.40

自引率

7.40%

发文量

949

期刊介绍： Science China Materials (SCM) is a globally peer-reviewed journal that covers all facets of materials science. It is supervised by the Chinese Academy of Sciences and co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China. The journal is jointly published monthly in both printed and electronic forms by Science China Press and Springer. The aim of SCM is to encourage communication of high-quality, innovative research results at the cutting-edge interface of materials science with chemistry, physics, biology, and engineering. It focuses on breakthroughs from around the world and aims to become a world-leading academic journal for materials science.