In Vitro Utilization of Prebiotics by Listeria monocytogenes

IF 4.1 2区生物学 Q2 MICROBIOLOGY

Microorganisms Pub Date : 2024-09-11 DOI:10.3390/microorganisms12091876

Tereza Kodešová, Anna Mašlejová, Eva Vlková, Šárka Musilová, Kristýna Horváthová, Hana Šubrtová Salmonová

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Abstract

Listeria monocytognes is an emerging pathogen responsible for the serious foodborne disease, listeriosis. The commensal gut microbiota is the first line of defense against pathogen internalization. The gut microbiome can be modified by prebiotic substrates, which are frequently added to food products and dietary supplements. Prebiotics should selectively support the growth of beneficial microbes and thus improve host health. Nevertheless, little is known about their effect on the growth of L. monocytogenes. The aim of this study was to evaluate the growth ability of four L. monocytogenes strains, representing the most common serotypes, on prebiotic oligosaccharides (beta-(1,3)-D-glucan, inulin, fructooligosaccharides, galactooligosaccharides, lactulose, raffinose, stachyose and 2′-fucosyllactose and a mixture of human milk oligosaccharides) as a sole carbon source. The results showed that only beta-(1,3)-D-glucan was metabolized by L. monocytogenes. These cell culture data suggest that beta-(1,3)-D-glucan may not be selectively utilized by healthy commensal bacteria, and its role in intestinal pathogen growth warrants further exploration in vivo.

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李斯特菌对益生元的体外利用

单核细胞李斯特菌是导致严重食源性疾病李斯特菌病的新病原体。共生的肠道微生物群是防止病原体内化的第一道防线。肠道微生物群可以通过益生元底物来改变，而益生元底物经常被添加到食品和膳食补充剂中。益生元应该有选择性地支持有益微生物的生长，从而改善宿主的健康。然而，人们对益生元对单核细胞增多性乳酸杆菌生长的影响知之甚少。本研究旨在评估代表最常见血清型的四种单核细胞增生梭菌菌株以益生元低聚糖（β-(1,3)-D-葡聚糖、菊粉、果寡糖、半乳寡糖、乳果糖、棉子糖、水苏糖和 2′-岩藻糖以及人乳低聚糖混合物）为唯一碳源的生长能力。结果表明，单核细胞增多性乳酸杆菌只代谢β-(1,3)-D-葡聚糖。这些细胞培养数据表明，β-(1,3)-D-葡聚糖可能不会被健康的共生菌选择性利用，其在肠道病原体生长中的作用值得在体内进一步探索。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.

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