DIB‐BOT: An Open‐Source Hardware Approach for Plate‐Integrated Droplet Interface Bilayer Deposition

IF 4.3 3区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Advanced Materials Interfaces Pub Date : 2024-09-02 DOI:10.1002/admi.202400413

Alexander F Mason, Shelley FJ Wickham, Matthew AB Baker

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Abstract

Droplet interface bilayers (DIBs) offer a controlled lipid environment for studying membrane‐bound processes, with applications in artificial cells, biosensing, and biophysics. Current DIB fabrication faces challenges due to time‐consuming processes and specialized equipment, limiting scale‐up and hindering statistical significance in single‐molecule assays. This research introduces “DIB‐BOT,” an open‐source solution combining a nanoinjector and a 3D printer. DIB‐BOT enables rapid, reproducible DIB fabrication, overcoming manual limitations. Using off‐the‐shelf components, DIB‐BOT ensures high spatial reproducibility, minimal user input, and scalable experiments. The system's utility is demonstrated through pairwise droplet assembly and a fluorescence plate‐reader assay. Compared to manual fabrication, DIB‐BOT shows a 10‐fold reduction in droplet volume error, a threefold reduction in positional error, and 100% droplet yield. This method lowers entry barriers to DIB research, expanding its applications and enhancing data quality.

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DIB-BOT：用于平板集成液滴界面双层沉积的开源硬件方法

液滴界面双层膜（DIB）为研究膜结合过程提供了可控的脂质环境，可应用于人工细胞、生物传感和生物物理等领域。目前的 DIB 制备面临着费时的工艺和专业设备带来的挑战，限制了规模的扩大，妨碍了单分子检测的统计意义。这项研究介绍了 "DIB-BOT"，一种结合了纳米注射器和三维打印机的开源解决方案。DIB-BOT 能够快速、可重复地制造 DIB，克服了手工操作的限制。DIB-BOT 使用现成的组件，可确保高度的空间可重复性、最少的用户输入和可扩展的实验。该系统的实用性通过成对液滴组装和荧光平板阅读器检测得到了验证。与手工制造相比，DIB-BOT 显示液滴体积误差减少了 10 倍，位置误差减少了 3 倍，液滴产量达到 100%。这种方法降低了 DIB 研究的入门门槛，扩大了其应用范围并提高了数据质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advanced Materials Interfaces CHEMISTRY, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

8.40

自引率

5.60%

发文量

1174

审稿时长

1.3 months

期刊介绍： Advanced Materials Interfaces publishes top-level research on interface technologies and effects. Considering any interface formed between solids, liquids, and gases, the journal ensures an interdisciplinary blend of physics, chemistry, materials science, and life sciences. Advanced Materials Interfaces was launched in 2014 and received an Impact Factor of 4.834 in 2018. The scope of Advanced Materials Interfaces is dedicated to interfaces and surfaces that play an essential role in virtually all materials and devices. Physics, chemistry, materials science and life sciences blend to encourage new, cross-pollinating ideas, which will drive forward our understanding of the processes at the interface. Advanced Materials Interfaces covers all topics in interface-related research: Oil / water separation, Applications of nanostructured materials, 2D materials and heterostructures, Surfaces and interfaces in organic electronic devices, Catalysis and membranes, Self-assembly and nanopatterned surfaces, Composite and coating materials, Biointerfaces for technical and medical applications. Advanced Materials Interfaces provides a forum for topics on surface and interface science with a wide choice of formats: Reviews, Full Papers, and Communications, as well as Progress Reports and Research News.