Polyherbal Antiacne Gel: In Vitro Antibacterial Activity and Efficacy Evaluation Against Cutibacterium acnes.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Assay and drug development technologies Pub Date : 2024-09-10 DOI:10.1089/adt.2024.031

Praveen Kumar Gaur,Rosaline Mishra,Rahul Kaushik,Krishan Kumar Verma,Nitin Kumar,Kank Lata

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引用次数: 0

Abstract

Acne is a common skin condition that affects people of all ages and can lead to significant physical and psychological distress. The first line of action against acne is topical products, though the most effective are topical antibiotics. In such a scenario, the development of effective and safe herbal formulations for the treatment of acne is of great importance. Rubia cordifolia, Aloe barbadensis, and Allium cepa extracts are rich sources of bioactive metabolites and are safe compared with antibiotics, in addition to being cost effective, sustainable, and eco-friendly. Also, their combination has not been studied for treating acne, and their potential benefits need to be investigated. The present study aimed to develop an effective polyherbal gel formulation of R. cordifolia, A. barbadensis, and A. cepa combined extract for treating acne and validate its effect with reference to conventional antibiotics. Plant materials were extracted in water by the reflux method, and phytochemical analysis was done for flavonoid, anthraquinone, and phenolic contents. The combined extract (R. cordifolia, A. barbadensis, and A. cepa extracts) was formulated in gel. The selected polyherbal gel was evaluated for in vitro antibacterial activity using agar well diffusion against Cutibacterium acnes (P. acnes) culture. Phytochemical analysis of the composite extract revealed the rich presence of flavonoids, phenolics, and anthraquinones. The polyherbal gels showed good physicochemical properties; however, FCEG-4 was selected for further studies. It was found to be effective against C. acnes (MTCC 1951) in agar well diffusion, as it showed a similar zone of inhibition as that of standard. Also, during in vivo studies, FCEG-4 showed comparable efficacy with clindamycin gel. It was concluded from the study that composite extracts incorporated in an aqueous-based gel system were effective in topical therapy of mild acne vulgaris, showing similar efficacy to that of clindamycin cream.

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多草本祛痘凝胶：针对痤疮杆菌的体外抗菌活性和功效评估

痤疮是一种常见的皮肤病，影响着各个年龄段的人，并可能导致严重的生理和心理困扰。治疗痤疮的第一线是外用产品，但最有效的是外用抗生素。在这种情况下，开发有效、安全的草药配方来治疗痤疮就显得尤为重要。茜草、芦荟和薤白提取物含有丰富的生物活性代谢物，与抗生素相比安全，而且具有成本效益、可持续性和生态友好性。此外，还没有研究过将它们结合起来治疗痤疮的方法，因此需要对它们的潜在益处进行研究。本研究旨在开发一种有效的多草本凝胶配方，将 R. cordifolia、A. barbadensis 和 A. cepa 联合提取用于治疗痤疮，并参照传统抗生素验证其效果。采用回流法对植物材料进行水提取，并对黄酮、蒽醌和酚含量进行植物化学分析。将混合提取物（R. cordifolia、A. barbadensis 和 A. cepa 提取物）配制成凝胶。采用琼脂井扩散法对所选的多草本凝胶进行了体外抗菌活性评估，以对抗痤疮棒状杆菌（P. acnes）培养物。复合提取物的植物化学分析显示，其中含有丰富的类黄酮、酚类和蒽醌类化合物。多草本凝胶显示出良好的理化特性；然而，FCEG-4 被选为进一步研究的对象。在琼脂井扩散试验中发现，FCEG-4 对痤疮丙酸杆菌（MTCC 1951）有效，其抑制区与标准品相似。此外，在体内研究中，FCEG-4 也显示出与克林霉素凝胶相当的功效。研究得出的结论是，水基凝胶系统中的复合提取物对轻度寻常痤疮的局部治疗有效，显示出与克林霉素乳膏相似的疗效。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Assay and drug development technologies 医学-生化研究方法

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ASSAY and Drug Development Technologies provides access to novel techniques and robust tools that enable critical advances in early-stage screening. This research published in the Journal leads to important therapeutics and platforms for drug discovery and development. This reputable peer-reviewed journal features original papers application-oriented technology reviews, topical issues on novel and burgeoning areas of research, and reports in methodology and technology application. ASSAY and Drug Development Technologies coverage includes: -Assay design, target development, and high-throughput technologies- Hit to Lead optimization and medicinal chemistry through preclinical candidate selection- Lab automation, sample management, bioinformatics, data mining, virtual screening, and data analysis- Approaches to assays configured for gene families, inherited, and infectious diseases- Assays and strategies for adapting model organisms to drug discovery- The use of stem cells as models of disease- Translation of phenotypic outputs to target identification- Exploration and mechanistic studies of the technical basis for assay and screening artifacts