Application of ATR-FTIR Spectroscopy for Analysis of Salt Stress in Brussels Sprouts

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-08-26 DOI:10.3390/metabo14090470

Su-Min Yun, Cheol-Soo Kim, Jeung-Joo Lee, Jung-Sung Chung

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引用次数: 0

Abstract

Salt stress is one of the environmental stresses that significantly reduces crop productivity and quality worldwide. Methods to overcome salt stress include developing salt-resistant crops by inserting various resistance genes or to diagnosing and responding to the effects of salt stress at an early stage. In this study, we investigate the effects of salinity stress on growth, photosynthetic efficiency, and metabolic changes in Brussels sprouts (Brassica oleracea var. gemmifera). Fresh weight and leaf area decreased significantly with increasing NaCl concentration, indicating that salinity stress has a detrimental effect on plant growth. However, chlorophyll fluorescence parameters did not show significant changes, suggesting that photosynthetic efficiency was not significantly affected over 10 days. Fourier transform infrared (FTIR) spectroscopy revealed notable metabolic adjustments, especially in lipids, plastids, proteins, and carbohydrates, indicating biosynthesis of protective compounds such as anthocyanins and proline in response to salinity stress. Pearson correlation analysis confirmed a strong relationship between NaCl concentration and the observed physiological and metabolic changes. The findings highlight the potential of FTIR spectroscopy as a non-destructive tool for early detection of salinity stress and timely intervention to improve crop resilience and yield. This study highlights the widespread application of FTIR spectroscopy in agricultural research to manage abiotic stresses in crops.

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应用 ATR-FTIR 光谱分析球芽甘蓝中的盐胁迫

盐胁迫是严重降低全球作物产量和质量的环境胁迫之一。克服盐胁迫的方法包括通过插入各种抗性基因来开发抗盐作物，或在早期诊断和应对盐胁迫的影响。本研究调查了盐胁迫对球芽甘蓝（Brassica oleracea var. gemmifera）生长、光合效率和代谢变化的影响。鲜重和叶面积随着 NaCl 浓度的增加而显著下降，表明盐胁迫对植物生长有不利影响。然而，叶绿素荧光参数并未出现明显变化，这表明光合作用效率在 10 天内并未受到明显影响。傅立叶变换红外光谱（FTIR）显示了显著的代谢调整，尤其是脂质、质体、蛋白质和碳水化合物，表明花青素和脯氨酸等保护性化合物的生物合成对盐胁迫做出了反应。皮尔逊相关分析证实，NaCl 浓度与观察到的生理和代谢变化之间存在密切关系。研究结果凸显了傅立叶变换红外光谱作为一种非破坏性工具的潜力，可用于早期检测盐渍胁迫并及时干预，以提高作物的抗逆性和产量。这项研究强调了傅立叶变换红外光谱技术在农业研究中的广泛应用，以管理作物的非生物胁迫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.