The Impact of Varying Pasture Levels on the Metabolomic Profile of Bovine Ruminal Fluid

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-08-28 DOI:10.3390/metabo14090476

Claire Connolly, Mark Timlin, Sean A. Hogan, Tom F. O’Callaghan, André Brodkorb, Michael O’Donovan, Deirdre Hennessy, Ellen Fitzpatrick, Kieran McCarthy, John P. Murphy, Lorraine Brennan

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引用次数: 0

Abstract

A pasture or concentrate-based dietary regime impacts a variety of factors including both ruminal health and function, and consequently milk production and quality. The objective of this study was to examine the effect of feeding differing pasture levels on the metabolite composition of bovine ruminal fluid. Ruminal fluid was obtained from rumen-cannulated spring-calving cows (N = 9, Holstein-Friesian breed, average lactation number = 5) fed one of three diets across a full lactation season. Group 1 (pasture) consumed perennial ryegrass supplemented with 5% concentrates; group 2 received a total mixed ration (TMR) diet; and group 3 received a partial mixed ration (PMR) diet which included pasture and a TMR. Samples were taken at two timepoints: morning and evening. Metabolomic analysis was performed using nuclear magnetic resonance (1H-NMR) spectroscopy. Statistical analysis revealed significant changes across the dietary regimes in both morning and evening samples, with distinct alterations in the metabolite composition of ruminal fluid from pasture-fed cows (FDR-adjusted p-value < 0.05). Acetate and butyrate were significantly higher in samples derived from a pasture-based diet whereas sugar-related metabolites were higher in concentrate-based samples. Furthermore, a distinct diurnal impact on the metabolite profile was evident. This work lays the foundation for understanding the complex interaction between dietary regime and ruminal health.

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不同牧草水平对牛胃液代谢组谱的影响

以牧草或精料为主的日粮制度会影响多种因素，包括瘤胃健康和功能，进而影响牛奶产量和质量。本研究旨在探讨饲喂不同水平的牧草对牛瘤胃液代谢物组成的影响。从瘤胃封存的春季犊牛（N = 9，荷斯坦-弗里斯兰品种，平均泌乳数 = 5）的瘤胃液中提取，在整个泌乳期喂食三种日粮中的一种。第 1 组（牧草）食用多年生黑麦草，辅以 5% 的精料；第 2 组食用全混合日粮 (TMR)；第 3 组食用部分混合日粮 (PMR)，其中包括牧草和全混合日粮。样品在两个时间点采集：早晨和傍晚。使用核磁共振（1H-NMR）光谱进行代谢组分析。统计分析表明，早晨和傍晚采样的奶牛瘤胃液中的代谢物组成发生了明显变化（经 FDR 调整的 p 值 < 0.05）。在以牧草为食的样本中，乙酸盐和丁酸盐含量明显较高，而在以浓缩饲料为食的样本中，与糖相关的代谢物含量较高。此外，代谢物的昼夜变化也很明显。这项研究为了解日粮制度与瘤胃健康之间复杂的相互作用奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.