The preventive effects of natural plant compound carvacrol against combined UVA and UVB-induced endoplasmic reticulum stress in skin damage of rats

IF 2.7 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Gulsah Evyapan, Halil Ozkol, Hatice Uce Ozkol, Özge Alvur, Sakine Akar
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Abstract

The skin is constantly exposed to a variety of environmental stressors, including ultraviolet (UV) radiation. Exposure of the skin to UV radiation causes a number of detrimental biological damages such as endoplasmic reticulum (ER) stress. The ER stress response is a cytoprotective mechanism that maintains homeostasis of the ER by increasing the capacity of the ER against the accumulation of unfolded proteins in the ER. Carvacrol (CRV) is a monoterpenoid phenol found in essential oils with antimicrobial and anti-inflammatory activities. We investigated for the first time in the literature the potential protective role of CRV against combined UVA and UVB-induced skin damage by targeting the ER stress pathway in a rat model. For this purpose, expressions of Grp78, Perk, Atf6, Ire-1, Chop, Xbp1, Casp12, elF2α, and Traf2 genes related to ER stress were analyzed by RT-PCR and protein expression levels of GRP78, ATF6, CHOP, and XBP1 were determined by ELISA assay in tissue sections taken from the back of the rats. As a result of analysis, it was seen that the expression levels of aforementioned ER stress genes increased significantly in the UVA + UVB irradiated group compared to the control group, while their expression levels decreased markedly by supplementation of CRV in UVA + UVB + CRV group. With regard to expressions of foregoing proteins, their levels escalated notably with UVA + UVB application and decreased markedly by CRV supplementation. In conclusion, present study revealed that CRV ameliorates UVA + UVB-induced ER stress via reducing the expression of mRNA as well as proteins involved in the unfolded protein response (UPR) pathway and inducing apoptosis as evidenced from high Caspase12 level.

Abstract Image

天然植物化合物香芹酚对 UVA 和 UVB 联合诱导的大鼠皮肤损伤内质网应激的预防作用
皮肤经常暴露在各种环境压力下,包括紫外线(UV)辐射。皮肤暴露在紫外线辐射下会造成一系列有害的生物损伤,如内质网(ER)应激。内质网应激反应是一种细胞保护机制,它通过提高内质网抵御未折叠蛋白质在内质网中积累的能力来维持内质网的平衡。香芹酚(CRV)是一种存在于精油中的单萜酚,具有抗菌和抗炎活性。我们首次在文献中研究了 CRV 在大鼠模型中通过靶向 ER 应激途径对 UVA 和 UVB 引起的皮肤损伤的潜在保护作用。为此,通过 RT-PCR 分析了大鼠背部组织切片中与 ER 应激相关的 Grp78、Perk、Atf6、Ire-1、Chop、Xbp1、Casp12、elF2α 和 Traf2 基因的表达,并通过 ELISA 检测确定了 GRP78、ATF6、CHOP 和 XBP1 的蛋白表达水平。分析结果表明,与对照组相比,UVA + UVB 照射组的上述ER应激基因的表达水平明显升高,而在 UVA + UVB + CRV 组中,补充 CRV 后,上述ER应激基因的表达水平明显降低。至于前述蛋白质的表达,它们的水平在应用 UVA + UVB 时明显升高,而在补充 CRV 后明显降低。总之,本研究揭示了 CRV 可通过减少参与未折叠蛋白反应(UPR)途径的 mRNA 和蛋白质的表达以及诱导细胞凋亡来改善 UVA + UVB 诱导的 ER 应激,Caspase12 的高水平证明了这一点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences 生物-生化与分子生物学
CiteScore
5.60
自引率
6.50%
发文量
201
审稿时长
2.3 months
期刊介绍: A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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