Supplementation with heat-killed Akkermansia muciniphila EB-AMDK19 counteracts diet-induced overweight in beagles.

IF 2 3区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Moon-Gi Hong,Yoonmi Lee,Won-Seok Chung,Jae-Gu Seo,Sang-Nam Lee
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Abstract

Obesity is a major health problem in dogs and is strongly associated with an increased risk of chronic inflammatory and metabolic diseases. The microaerophilic human gut bacterium Akkermansia muciniphila has been proposed as a potential preventive and therapeutic agent against obesity in both humans and mice; however, the protective effects of human-derived A. muciniphila against canine obesity remain unstudied. We previously demonstrated that the heat-killed A. muciniphila strain EB-AMDK19 (AMDK19-HK) isolated from the faeces of a healthy Korean exerts similar protective effects as the live bacterium in mice with high-fat-diet (HFD)-induced obesity. Here, we evaluated the effects of AMDK19-HK on body weight, body fat mass, haematological and biochemical parameters, and faecal microbiota composition in beagles fed an HFD for 12 weeks. AMDK19-HK supplementation effectively suppressed body weight increase, body fat deposition and serum triglyceride increase in the canine model; however, no significant changes in the overall haematological and biochemical parameters were observed, reflecting the direct anti-obesity effect of AMDK19-HK. Additionally, 16S rRNA gene sequencing revealed that AMDK19-HK supplementation induced significant changes in the faecal bacterial community, with an increased abundance of Firmicutes and a decreased abundance of Bacteroidota. These results suggest that AMDK19-HK can be used as a dietary supplement to counteract diet-induced overweight in dogs.
补充热处理杀死的Akkermansia muciniphila EB-AMDK19可抵消饮食引起的小猎犬超重。
肥胖是狗的一个主要健康问题,与慢性炎症和代谢疾病风险的增加密切相关。嗜微气的人类肠道细菌 Akkermansia muciniphila 被认为是预防和治疗人类和小鼠肥胖症的潜在药物;然而,源自人类的 A. muciniphila 对犬类肥胖症的保护作用仍未得到研究。我们以前曾证实,从健康韩国人粪便中分离出的热处理杀死的粘液噬菌体菌株 EB-AMDK19(AMDK19-HK)对高脂饮食(HFD)诱导肥胖的小鼠具有与活菌相似的保护作用。在这里,我们评估了 AMDK19-HK 对以高脂饮食喂养 12 周的小猎犬的体重、体脂量、血液学和生化指标以及粪便微生物群组成的影响。补充 AMDK19-HK 能有效抑制犬模型体重增加、体脂沉积和血清甘油三酯升高,但总体血液学和生化指标未观察到显著变化,这反映了 AMDK19-HK 的直接抗肥胖作用。此外,16S rRNA 基因测序显示,补充 AMDK19-HK 会诱导粪便细菌群落发生重大变化,其中固着菌的数量增加,类杆菌的数量减少。这些结果表明,AMDK19-HK可作为一种膳食补充剂,用于对抗饮食引起的犬超重。
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来源期刊
Archives of Animal Nutrition
Archives of Animal Nutrition 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
31
审稿时长
>24 weeks
期刊介绍: Archives of Animal Nutrition is an international journal covering the biochemical and physiological basis of animal nutrition. Emphasis is laid on original papers on protein and amino acid metabolism, energy transformation, mineral metabolism, vitamin metabolism, nutritional effects on intestinal and body functions in combination with performance criteria, respectively. It furthermore deals with recent developments in practical animal feeding, feedstuff theory, mode of action of feed additives, feedstuff preservation and feedstuff processing. The spectrum covers all relevant animal species including food producing and companion animals, but not aquatic species. Seldom can priority be given to papers covering more descriptive studies, even if they may be interesting and technically sound or of impact for animal production, or for topics of relevance for only particular regional conditions.
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