Simultaneous identification of several Helicobacter pylori virulence factors based on chitosan@ionic liquid and graphitic carbon nitride modified glassy carbon microspheres ionic liquid paste electrode

IF 2.4 4区化学 Q3 CHEMISTRY, PHYSICAL

Ionics Pub Date : 2024-09-07 DOI:10.1007/s11581-024-05804-x

Wenli Qiao, Zhen Zhang, Xiying Tang, Mengjun Chang, Le Guo, Xinsheng Liu, Yonghong Li

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Abstract

Helicobacter pylori (H. pylori) can cause a variety of gastric diseases, such as chronic gastritis, gastric ulcer, intestinal metaplasia, and dysplasia, and eventually lead to gastric cancer. Therefore, it is crucial to achieve early diagnosis of H. pylori. The biosensing platform was constructed for Epitope Vaccine against Four Virulence Proteins from H. pylori (FVpE) based on chitosan (CS)@ionic liquid (IL) and graphitic carbon nitride (g-C₃N₄) modified glassy carbon microspheres ionic liquid paste electrode. The results showed that the substrate electrode had good electrical conductivity, which could amplify the response signal and improve the detection sensitivity. The developed immunosensor exhibited good immunoreactivity for H. pylori antibodies with wide linear range (0.05–5 ng mL⁻¹) and low detection limit (0.01 ng mL⁻¹). In addition, the electrochemical immunosensor presented quick response, high sensitivity, good specificity, and stability. The immunosensor could be used to detect H. pylori in serum samples with recoveries between 99 and 112%. It can provide a theoretical basis for the development of label-free electrochemical H. pylori immunosensors.

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基于壳聚糖@离子液体和石墨氮化碳修饰玻璃碳微球离子液体糊状电极的幽门螺杆菌致病因子的同时鉴定

幽门螺杆菌（H. pylori）可引起多种胃病，如慢性胃炎、胃溃疡、肠化生和发育不良，并最终导致胃癌。因此，实现幽门螺杆菌的早期诊断至关重要。基于壳聚糖（CS）@离子液体（IL）和氮化石墨碳（g-C3N4）修饰的玻璃碳微球离子液体浆电极，构建了针对幽门螺杆菌四种病毒蛋白的表位疫苗（FVpE）的生物传感平台。结果表明，基底电极具有良好的导电性，可以放大响应信号，提高检测灵敏度。所开发的免疫传感器对幽门螺杆菌抗体具有良好的免疫反应性，线性范围宽（0.05-5 ng mL-1），检测限低（0.01 ng mL-1）。此外，该电化学免疫传感器反应快、灵敏度高、特异性好且稳定。该免疫传感器可用于检测血清样本中的幽门螺杆菌，回收率在 99% 至 112% 之间。它为开发无标记的电化学幽门螺杆菌免疫传感器提供了理论依据。

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来源期刊

Ionics 化学-电化学

CiteScore

5.30

自引率

7.10%

发文量

427

审稿时长

2.2 months

期刊介绍： Ionics is publishing original results in the fields of science and technology of ionic motion. This includes theoretical, experimental and practical work on electrolytes, electrode, ionic/electronic interfaces, ionic transport aspects of corrosion, galvanic cells, e.g. for thermodynamic and kinetic studies, batteries, fuel cells, sensors and electrochromics. Fast solid ionic conductors are presently providing new opportunities in view of several advantages, in addition to conventional liquid electrolytes.

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