Transcription factor DcbZIPs regulate secondary metabolism in Dendrobium catenatum during cold stress

IF 5.4 2区生物学 Q1 PLANT SCIENCES

Physiologia plantarum Pub Date : 2024-09-11 DOI:10.1111/ppl.14501

Xiaohui Zhou, Chenfei Lu, Fenfen Zhou, Yanqin Zhu, Wu Jiang, Aicun Zhou, Yanghui Shen, Lanying Pan, Aimin Lv, Qingsong Shao

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引用次数: 0

Abstract

Cold stress seriously affects plant development and secondary metabolism. The basic region/leucine zipper (bZIP) is one of the largest transcription factor (TFs) family and widely involved in plant cold stress response. However, the function of bZIP in Dendrobium catenatum has not been well‐documented. Cold inhibited the growth of D. catenatum and increased total polysaccharide and alkaloid contents in stems. Here, 62 DcbZIP genes were identified in D. catenatum, which were divided into 13 subfamilies. Among them, 58 DcbZIPs responded to cold stress, which were selected based on the transcriptome database produced from cold‐treated D. catenatum seedlings. Specifically, the expression of DcbZIP3/6/28 was highly induced by cold treatment in leaves or stems. Gene sequence analysis indicated that DcbZIP3/6/28 contains the bZIP conserved domain and is localized to the cell nucleus. Co‐expression networks showed that DcbZIP6 was significantly negatively correlated with PAL2 (palmitoyl‐CoA), which is involved in flavonoid metabolism. Moreover, DcbZIP28 has significant negative correlations with various metabolism‐related genes in the polysaccharide metabolic pathway, including PFKA1 (6‐phosphofructokinase), ALDO2 (aldose‐6‐phosphate reductase) and SCRK5 (fructokinase). These results implied that DcbZIP6 or DcbZIP28 are mainly involved in flavonoid or polysaccharide metabolism. Overall, these findings provide new insights into the roles of the DcbZIP gene family in secondary metabolism in D. catenatum under cold stress.

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转录因子 DcbZIPs 在冷胁迫期间调控铁皮石斛的次生代谢

冷胁迫严重影响植物的生长发育和次生代谢。基本区/亮氨酸拉链（bZIP）是最大的转录因子（TFs）家族之一，广泛参与植物的冷胁迫响应。然而，bZIP 在铁皮石斛中的功能尚未得到充分证实。寒冷抑制了铁皮石斛的生长，并增加了茎中总多糖和生物碱的含量。本文鉴定了 62 个 DcbZIP 基因，并将其分为 13 个亚科。其中，58个DcbZIP基因对冷胁迫有响应，这些基因是根据冷处理D. catenatum幼苗产生的转录组数据库筛选出来的。具体而言，DcbZIP3/6/28在叶片或茎中的表达受到冷处理的高度诱导。基因序列分析表明，DcbZIP3/6/28 包含 bZIP 保守结构域，定位于细胞核。共表达网络显示，DcbZIP6与参与类黄酮代谢的PAL2（棕榈酰-CoA）呈显著负相关。此外，DcbZIP28与多糖代谢途径中的多个代谢相关基因，包括PFKA1（6-磷酸果糖激酶）、ALDO2（醛糖-6-磷酸还原酶）和SCRK5（果糖激酶）呈显著负相关。这些结果表明，DcbZIP6 或 DcbZIP28 主要参与类黄酮或多糖代谢。总之，这些研究结果为了解 D. catenatum 在冷胁迫下 DcbZIP 基因家族在次生代谢中的作用提供了新的视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiologia plantarum 生物-植物科学

CiteScore

11.00

自引率

3.10%

发文量

224

审稿时长

3.9 months

期刊介绍： Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.

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