Evaluation of Burn Wound Healing Efficacy and Biocompatibility of Centella asiatica Mediated Synthesised AgNPs Loaded Hybrid Nanofiber Scaffold: In Vitro and In Vivo Studies

IF 4.2 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Macromolecular Materials and Engineering Pub Date : 2024-08-18 DOI:10.1002/mame.202400186

Ogün Bozkaya, Esra Bozkaya, Hüsamettin Ekici, Mehmet Eray Alçığır, Yaşar Şahin, Nebahat Aytuna Çerçi, Siyami Karahan, Mustafa Yiğitoğlu, İbrahim Vargel

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Abstract

The aim of this study is to evaluate the cell responses, potential skin reactions during the treatment process and burn wound healing efficacy of electrospun polycaprolactone/polyethylene oxide (PCL/PEO) nanofibers (NFs) containing Centella asiatica mediated synthesized silver nanoparticles (CA-AgNPs) by in vitro and in vivo studies. Apoptosis-necrosis, genotoxicity, hemolysis, and cell attachment studies are carried out within the scope of in vitro tests, and irritation, sensitivity, and burn wound studies are carried out within the scope of in vivo tests. The apoptotic index value of CA-AgNPs-[PCL/PEO] NFs material on L929 fibroblast cells is determined as 5.0 ± 1.0% at the highest concentration and the necrotic index value is 5.0 ± 0.3%. Micronucleus rates (%) of NFs treated with CHO (Chinese Hamster Ovary) cells are not at genotoxic level. The hemolytic index value of NFs dressing is determined as 0.23 ± 0.03%, The primary irritation index (PII) value of NFs wound dressing is calculated as 0.36 by irritation tests. In addition, the potential sensitization reaction of NFs extract on guinea pigs is evaluated and the sensitization score is determined as 0.9. The healing efficacy of NFs material on second-degree burn wounds compared to a commercial product is supported by pathomorphological findings.

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评估积雪草介导合成的 AgNPs 负载混合纳米纤维支架的烧伤伤口愈合功效和生物相容性：体外和体内研究

本研究旨在通过体外和体内研究，评估含有积雪草介导合成的银纳米粒子（CA-AgNPs）的电纺聚己内酯/聚环氧乙烷（PCL/PEO）纳米纤维（NFs）在治疗过程中的细胞反应、潜在皮肤反应和烧伤创面愈合效果。体外试验范围包括细胞凋亡-坏死、遗传毒性、溶血和细胞附着研究，体内试验范围包括刺激性、敏感性和烧伤研究。在最高浓度下，CA-AgNPs-[PCL/PEO] NFs 材料对 L929 成纤维细胞的凋亡指数值为 5.0 ± 1.0%，坏死指数值为 5.0 ± 0.3%。用 CHO（中国仓鼠卵巢）细胞处理的 NFs 微核率（%）未达到遗传毒性水平。NFs 敷料的溶血指数值为 0.23 ± 0.03%，通过刺激试验计算出 NFs 伤口敷料的一级刺激指数 (PII) 值为 0.36。此外，还评估了 NFs 提取物对豚鼠的潜在致敏反应，确定其致敏指数为 0.9。病理形态学研究结果支持 NFs 材料对二度烧伤创面的愈合效果优于商业产品。

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来源期刊

Macromolecular Materials and Engineering 工程技术-材料科学：综合

CiteScore

7.30

自引率

5.10%

发文量

328

审稿时长

1.6 months

期刊介绍： Macromolecular Materials and Engineering is the high-quality polymer science journal dedicated to the design, modification, characterization, processing and application of advanced polymeric materials, including membranes, sensors, sustainability, composites, fibers, foams, 3D printing, actuators as well as energy and electronic applications. Macromolecular Materials and Engineering is among the top journals publishing original research in polymer science. The journal presents strictly peer-reviewed Research Articles, Reviews, Perspectives and Comments. ISSN: 1438-7492 (print). 1439-2054 (online). Readership:Polymer scientists, chemists, physicists, materials scientists, engineers Abstracting and Indexing Information: CAS: Chemical Abstracts Service (ACS) CCR Database (Clarivate Analytics) Chemical Abstracts Service/SciFinder (ACS) Chemistry Server Reaction Center (Clarivate Analytics) ChemWeb (ChemIndustry.com) Chimica Database (Elsevier) COMPENDEX (Elsevier) Current Contents: Physical, Chemical & Earth Sciences (Clarivate Analytics) Directory of Open Access Journals (DOAJ) INSPEC (IET) Journal Citation Reports/Science Edition (Clarivate Analytics) Materials Science & Engineering Database (ProQuest) PASCAL Database (INIST/CNRS) Polymer Library (iSmithers RAPRA) Reaction Citation Index (Clarivate Analytics) Science Citation Index (Clarivate Analytics) Science Citation Index Expanded (Clarivate Analytics) SciTech Premium Collection (ProQuest) SCOPUS (Elsevier) Technology Collection (ProQuest) Web of Science (Clarivate Analytics)