Dissemination of blaNDM-5 Driven by Horizontal Transfer of IncFIA Plasmid Between Escherichia coli and Klebsiella pneumoniae Co-Isolated from a Patient’s Ascitic Fluid

IF 2.9 3区医学 Q2 INFECTIOUS DISEASES

Infection and Drug Resistance Pub Date : 2024-09-13 DOI:10.2147/idr.s478304

Jing Yu, Yanzi Ding, Xue Zhang, Shuhong Tai, Chengwen Zhang, Cailin Liu, Enwu Yuan, Yitao Duan

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Abstract

Purpose: Understanding the horizontal transfer of resistance genes, such as bla_NDM-5, is pivotal in developing strategies to control the spread of resistance. In this study, we isolated two bacterial strains, Escherichia coli (designated GYB01) and Klebsiella pneumoniae (designated GYB02), from a single patient. The aim of our research is to explore the biological characteristics of these strains and to investigate the interspecies horizontal transfer of bla_NDM-5.
Materials and Methods: Strain identification and antimicrobial susceptibility testing were conducted using the Vitek 2 system. Both GYB01 and GYB02 were sequenced with the Illumina HiSeq platform. Bioinformatics analysis tools, including multilocus sequence typing, PlasmidFinder, ResFinder, and others, were utilized to analyze the strains. Additionally, conjugation assays and Galleria mellonella infection assays were employed to assess the strains.
Results: The isolates exhibited similar antimicrobial resistance profiles and both harbored the bla_NDM-5 gene within the IncFIA plasmids (pGYB01-2, 165.8 kb and pGYB02-2, 211.6 kb, respectively). These plasmids (pGYB01-2 and pGYB02-2) shared over 99% homology, suggesting a common ancestral origin. Conjugation experiments confirmed the transferability of the bla_NDM-5 carrying IncFIA plasmids among Enterobacteriaceae. GYB02 possessed an iucACD-iutA gene cluster, exhibited high virulence, and tested positive in the string test.
Conclusion: Our findings provide direct evidence of potential in vivo interspecies transfer of a multidrug-resistant plasmid, thus enriching our understanding of the mechanisms driving multidrug resistance (MDR) and aiding in the formulation of containment and treatment strategies.

Keywords: CRE, NDM-5 carbapenemase, IncFIA plasmid, interspecies horizontal transfer

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从患者腹腔积液中分离出的大肠埃希菌和肺炎克雷伯菌共分离出的 IncFIA 质粒之间的水平转移驱动了 blaNDM-5 的传播

目的：了解耐药性基因（如 blaNDM-5）的水平转移对于制定控制耐药性传播的策略至关重要。在本研究中，我们从一名患者身上分离出了两株细菌，即大肠埃希菌（命名为 GYB01）和肺炎克雷伯菌（命名为 GYB02）。我们的研究旨在探索这些菌株的生物学特性，并研究 blaNDM-5 的种间水平转移：使用 Vitek 2 系统进行菌株鉴定和抗菌药敏感性测试。利用 Illumina HiSeq 平台对 GYB01 和 GYB02 进行了测序。生物信息学分析工具（包括多焦点序列分型、PlasmidFinder、ResFinder 等）被用来分析菌株。此外，还采用了共轭测定法和黑僵菌感染测定法来评估菌株：结果：分离菌株表现出相似的抗菌谱，并且都在 IncFIA 质粒（pGYB01-2，165.8 kb；pGYB02-2，211.6 kb）中携带 blaNDM-5 基因。这些质粒（pGYB01-2 和 pGYB02-2）的同源性超过 99%，表明它们有共同的祖先起源。共轭实验证实了携带 IncFIA 的 blaNDM-5 质粒在肠杆菌科细菌中的可转移性。GYB02 具有 iucACD-iutA 基因簇，表现出很强的毒力，并在串联测试中呈阳性：结论：我们的研究结果提供了耐多药质粒可能在体内种间转移的直接证据，从而丰富了我们对多药耐药性（MDR）驱动机制的认识，并有助于遏制和治疗策略的制定：CRE、NDM-5碳青霉烯酶、IncFIA质粒、种间水平转移

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来源期刊

Infection and Drug Resistance Medicine-Pharmacology (medical)

CiteScore

5.60

自引率

7.70%

发文量

826

审稿时长

16 weeks

期刊介绍： About Journal Editors Peer Reviewers Articles Article Publishing Charges Aims and Scope Call For Papers ISSN: 1178-6973 Editor-in-Chief: Professor Suresh Antony An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.

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