Sekgetho C. Mokwatlo , Bruno C. Klein , Pahola Thathiana Benavides , Eric C. D. Tan , Colin M. Kneucker , Chen Ling , Christine A. Singer , Robert Lyons , Violeta Sànchez i Nogué , Kelley V. Hestmark , Morgan A. Ingraham , Kelsey J. Ramirez , Christopher W. Johnson , Gregg T. Beckham , Davinia Salvachúa
{"title":"Bioprocess development and scale-up for cis,cis-muconic acid production from glucose and xylose by Pseudomonas putida†","authors":"Sekgetho C. Mokwatlo , Bruno C. Klein , Pahola Thathiana Benavides , Eric C. D. Tan , Colin M. Kneucker , Chen Ling , Christine A. Singer , Robert Lyons , Violeta Sànchez i Nogué , Kelley V. Hestmark , Morgan A. Ingraham , Kelsey J. Ramirez , Christopher W. Johnson , Gregg T. Beckham , Davinia Salvachúa","doi":"10.1039/d4gc03424d","DOIUrl":null,"url":null,"abstract":"<div><div> <em>cis</em>,<em>cis</em>-Muconic acid (MA) is a bio-based chemical that can be converted to direct replacement chemicals or performance-advantaged bioproducts. We recently engineered the bacterium <em>Pseudomonas putida</em> KT2440 for the co-utilization of glucose and xylose to produce MA. This study evaluates the effect of additional genetic modifications, media composition, and bioprocess strategy on MA titer, productivity, and yield in bioreactor cultivations. We achieve a MA titer of 47.2 g L<sup>−1</sup>, a productivity of 0.49 g L<sup>−1</sup> h<sup>−1</sup>, and a yield of 0.50 C-mol C-mol<sup>−1</sup> from glucose and xylose supplemented with 5% (v/v) corn steep liquor with a <em>P. putida</em> strain harboring the deletion of <em>gacS</em>. Additionally, we demonstrate efficient MA production from corn stover-derived sugars and scalability to 150 L bioreactors. Techno-economic analysis and life cycle assessment predict that adipic acid, derived from catalytic hydrogenation of MA, can achieve a selling price as low as $2.60 per kg, approaching cost parity and reducing greenhouse gas emissions by up to 80% relative to fossil carbon-based adipic acid.</div></div>","PeriodicalId":78,"journal":{"name":"Green Chemistry","volume":"26 19","pages":"Pages 10152-10167"},"PeriodicalIF":9.3000,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/gc/d4gc03424d?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Green Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/org/science/article/pii/S146392622400760X","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
cis,cis-Muconic acid (MA) is a bio-based chemical that can be converted to direct replacement chemicals or performance-advantaged bioproducts. We recently engineered the bacterium Pseudomonas putida KT2440 for the co-utilization of glucose and xylose to produce MA. This study evaluates the effect of additional genetic modifications, media composition, and bioprocess strategy on MA titer, productivity, and yield in bioreactor cultivations. We achieve a MA titer of 47.2 g L−1, a productivity of 0.49 g L−1 h−1, and a yield of 0.50 C-mol C-mol−1 from glucose and xylose supplemented with 5% (v/v) corn steep liquor with a P. putida strain harboring the deletion of gacS. Additionally, we demonstrate efficient MA production from corn stover-derived sugars and scalability to 150 L bioreactors. Techno-economic analysis and life cycle assessment predict that adipic acid, derived from catalytic hydrogenation of MA, can achieve a selling price as low as $2.60 per kg, approaching cost parity and reducing greenhouse gas emissions by up to 80% relative to fossil carbon-based adipic acid.
顺式粘多糖酸(MA)是一种生物基化学品,可直接转化为替代化学品或性能优越的生物产品。我们最近改造了假单胞菌 KT2440,使其能够共同利用葡萄糖和木糖生产 MA。本研究评估了生物反应器培养中额外的基因修饰、培养基组成和生物工艺策略对 MA 滴度、生产率和产量的影响。我们利用缺失 gacS 的 P. putida 菌株,在添加了 5%(v/v)玉米浸液的葡萄糖和木糖培养基中,实现了 47.2 g L-1 的 MA 滴度、0.49 g L-1 h-1 的生产率和 0.50 C-mol C-mol-1 的产量。此外,我们还展示了利用玉米秸秆衍生糖生产 MA 的高效性以及在 150 升生物反应器中的可扩展性。技术经济分析和生命周期评估预测,从催化氢化 MA 中提取的己二酸售价可低至每公斤 2.60 美元,接近成本平价,与化石碳基己二酸相比,可减少高达 80% 的温室气体排放。
期刊介绍:
Green Chemistry is a journal that provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998), which defines green chemistry as the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry aims to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. The journal welcomes submissions on all aspects of research relating to this endeavor and publishes original and significant cutting-edge research that is likely to be of wide general appeal. For a work to be published, it must present a significant advance in green chemistry, including a comparison with existing methods and a demonstration of advantages over those methods.