Insect cell production of chimeric virus-like particles based on human immunodeficiency virus GAG proteins and yellow fever virus envelope protein

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Fabricio da Silva Morgado, Roberta Cahú, Daniela Carrilho de Jesus, Lorena Carvalho de Souza Chaves, Bergmann Morais Ribeiro
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Abstract

The yellow fever virus (YFV) is a single stranded RNA virus belonging to the genus Orthoflavivirus that is capable of zoonotic transmissions that infect nonhuman and human primates. It is endemic in Brazil with recurrent epidemics of the disease, and it is transmitted through mosquitoes. The detection and immunization against YFV and other flaviviruses are fundamental for the management of the impacts of the disease in human environments. In an ongoing effort to develop new approaches for diagnostics and immunizations, we expressed VLPs displaying the yellow fever virus envelope protein (YFE) using recombinant baculovirus in insect cells. By co-expressing HIV-1 Pr55Gag protein (GAG) together with YFE we were able to generate chimeric VLPs containing a GAG core together with an envelope containing the YFE protein. The YFE and the chimeric GAG-YFE VLPs have potential as vaccine candidates and as reagents for serological assays in the detection of these viruses in human sera.

Abstract Image

用昆虫细胞生产基于人类免疫缺陷病毒 GAG 蛋白和黄热病病毒包膜蛋白的嵌合病毒样颗粒
黄热病病毒(YFV)是一种单股 RNA 病毒,属于正黄热病病毒属,能够通过人畜共患病传播,感染非人类和人类灵长类动物。它是巴西的地方病,经常流行,通过蚊子传播。检测YFV和其他黄病毒并对其进行免疫接种是控制该疾病对人类环境影响的基础。为了不断开发新的诊断和免疫方法,我们利用重组杆状病毒在昆虫细胞中表达了显示黄热病病毒包膜蛋白(YFE)的 VLPs。通过将 HIV-1 Pr55Gag 蛋白(GAG)与 YFE 共同表达,我们生成了含有 GAG 核心和含有 YFE 蛋白的包膜的嵌合 VLPs。YFE 和嵌合 GAG-YFE VLPs 有潜力成为候选疫苗,并可作为检测人类血清中这些病毒的血清学试剂。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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