Green synthesis and characterization studies of TiO2 nanoparticles and its potential biological performance

IF 5.45 Q1 Physics and Astronomy
R. Ramya, G. Muthulakshmi, S. Sudhahar, A. Bhaskaran
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引用次数: 0

Abstract

Recent interest was been sparked by the potential of eco-friendly nanomaterials against human pathogenic bacteria’s and cancer cells. In the first method, TiO was obtained from green synthesis method by using seed extraction. In the second method, TiO nanoparticles were chemically synthesized by using the sol-gel process. Powder XRD, UV-Vis, ATR-IR, FESEM with EDAX, and HR-TEM were used to analyze the characteristics of the synthesized TiO nanoparticles. XRD results showed the well-developed crystallized smaller particles. The sizes of the crystalline particles were estimated by using Size-Strain Plot (SSP). The disc diffusion method was used to examine the antibacterial activity of TiO nanoparticles against gram bacteria. The synthesized TiO nanoparticles showed the largest inhibition zone and found to be more antibacterial against gram-negative bacteria. When using the green synthesized nanoparticles at a dose of 1000 µg/mL, the largest zone of inhibition against measured 15 mm. Then, the chemically synthesized TiO had the same concentration exhibited 7 mm inhibition zone. Cytotoxicity activities of human breast cancer cell were evolved by using MTT assay and their observation were showed more efficient cytotoxicity of green synthesized TiO nanoparticles compared to the chemically synthesized TiO nanoparticles. The antioxidant activity potential of green and chemically synthesized titanium dioxide nanoparticles was examined and the maximum inhibition in 400 µg/mL concentrations was reported to be 53 % and 45 %, respectively. Bovine Serum Albumin (BSA) denaturation technique revealed the significant anti-inflammatory action of green synthesized TiO, with protein denaturation of egg albumin determined as 92.2 % at 400 µg/mL concentration.
TiO2 纳米粒子的绿色合成和表征研究及其潜在的生物性能
环保型纳米材料对人类致病菌和癌细胞的潜在抗性最近引发了人们的兴趣。在第一种方法中,TiO 是通过种子提取的绿色合成方法获得的。第二种方法是利用溶胶-凝胶工艺化学合成 TiO 纳米粒子。粉末 XRD、紫外可见光、ATR-IR、带 EDAX 的 FESEM 和 HR-TEM 被用来分析合成的 TiO 纳米粒子的特性。XRD 结果表明结晶较小的颗粒发育良好。利用尺寸-应变图(SSP)估算了结晶颗粒的尺寸。采用圆盘扩散法检测了 TiO 纳米粒子对革兰氏细菌的抗菌活性。合成的 TiO 纳米粒子显示出最大的抑菌区,对革兰氏阴性菌具有更强的抗菌能力。当使用绿色合成纳米粒子的剂量为 1000 微克/毫升时,测得的最大抑菌区为 15 毫米。而相同浓度的化学合成 TiO 的抑菌面积为 7 毫米。用 MTT 法检测了人乳腺癌细胞的细胞毒性,结果表明绿色合成的 TiO 纳米粒子比化学合成的 TiO 纳米粒子具有更有效的细胞毒性。研究还检测了绿色合成纳米二氧化钛和化学合成纳米二氧化钛的抗氧化活性潜力,结果表明,在 400 µg/mL 浓度下的最大抑制率分别为 53% 和 45%。牛血清白蛋白(BSA)变性技术揭示了绿色合成二氧化钛的显著抗炎作用,在 400 µg/mL 浓度下,鸡蛋白蛋白的蛋白质变性率为 92.2%。
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来源期刊
Nano-Structures & Nano-Objects
Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics
CiteScore
9.20
自引率
0.00%
发文量
60
审稿时长
22 days
期刊介绍: Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .
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