Potential in vitro antibacterial and anticancer properties of biosynthesized multifunctional silver nanoparticles using Martynia annua L. leaf extract

IF 5.45 Q1 Physics and Astronomy

Nano-Structures & Nano-Objects Pub Date : 2024-09-04 DOI:10.1016/j.nanoso.2024.101320

Megha B. Abbigeri, Bothe Thokchom, Santosh Mallikarjun Bhavi, Sapam Riches Singh, Pooja Joshi, Ramesh Babu Yarajarla

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引用次数: 0

Abstract

L., a medicinal herb rich in secondary metabolites, serves as a sustainable source for synthesizing silver nanoparticles (AgNPs). This study explores the eco-friendly production of AgNPs using aqueous leaf extract from and evaluates their biomedical applications. Characterization techniques including UV-Visible spectroscopy, FT-IR, SEM, TEM, and XRD confirm the spherical shape and FCC structure of the AgNPs, with a mean size of ∼11 nm and a surface charge of −24.4 mV. Biologically, the AgNPs exhibit potent antibacterial activity against both Gram positive and Gram negative bacteria, with dosage-dependent inhibition zones. AgNPs exhibited zone of inhibition comparable to those of standard antibiotic, penicillin, against Gram positive bacteria ( 22.09 mm and 23.72 mm) and Gram negative bacteria (, 13.82 mm and , 14.81 mm). They demonstrate cytotoxicity against NIH 3T3 and MCF-7 cells, with IC of 23.46 μg mL and 19.15 μg mL in cytotoxicity and anticancer assays, respectively. Notably, fragmentation assays reveal smear formation, indicating potential for inducing apoptosis. The synthesized AgNPs from exhibit spherical morphology, moderate stability, and significant antibacterial and anticancer properties, suggesting their potential as versatile biomedical agents.

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利用茉莉花叶提取物生物合成的多功能银纳米粒子的潜在体外抗菌和抗癌特性

L.是一种富含次生代谢产物的药草，是合成银纳米粒子（AgNPs）的可持续来源。本研究探讨了如何利用水性叶提取物以生态友好的方式生产 AgNPs，并对其生物医学应用进行了评估。包括紫外可见光谱、傅立叶变换红外光谱、扫描电镜、电子显微镜和 XRD 在内的表征技术证实了 AgNPs 的球形和 FCC 结构，其平均尺寸为 11 nm，表面电荷为 -24.4 mV。从生物学角度来看，AgNPs 对革兰氏阳性和革兰氏阴性细菌都有很强的抗菌活性，抑菌区的大小与剂量有关。AgNPs 对革兰氏阳性菌（22.09 毫米和 23.72 毫米）和革兰氏阴性菌（13.82 毫米和 14.81 毫米）的抑菌区与标准抗生素青霉素的抑菌区相当。它们对 NIH 3T3 和 MCF-7 细胞具有细胞毒性，在细胞毒性和抗癌试验中的 IC 值分别为 23.46 μg mL 和 19.15 μg mL。值得注意的是，碎裂试验显示了涂片的形成，表明其具有诱导细胞凋亡的潜力。合成的 AgNPs 呈球形，稳定性适中，具有显著的抗菌和抗癌特性，表明它们具有作为多功能生物药剂的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

22 days

期刊介绍： Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .