Profile and antimicrobial susceptibility patterns of bacteria isolated from effluents of Kolladiba and Debark hospitals

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-11 DOI:10.1515/biol-2022-0960

Tamene Milkessa Jiru, Ewunetu Ayanaw

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Abstract

This study aimed to investigate the presence of antibiotic susceptibility patterns and bacterial profiles of some multi-drug-resistant bacteria isolated from the effluents of Kolladiba and Debark Hospitals. Sixteen samples were collected from Kolladiba and Debark Hospitals in North Gondar, Ethiopia, to investigate the presence of multi-drug-resistant bacteria. To assess susceptibility patterns, well-isolated bacterial colonies were subjected to seven antibiotics. The selected resistant isolates were characterized using morphological and biochemical tests. Plasmid DNA analysis of the isolates was also performed. Out of a total of 28 bacterial isolates, 12 were found to be multi-drug resistant. Among the tested antibiotics, erythromycin was the most resistant antibiotic, while novobiocin was the most effective antibiotic. A plasmid profile study of the isolates revealed both the presence and absence of plasmids. The number of plasmids ranged from zero to four, with plasmid sizes of 100, 900, 1,000, 1,400, 1,500, and 1,800 base pairs. This study concluded that effluents from both hospitals have high number of multi-drug-resistant isolates. The genes responsible for multi-drug resistance in bacterial isolates under this study could be either plasmid-mediated or chromosomal DNA-mediated. The presence of multi-drug-resistant bacteria in these effluents should not be overlooked.

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从 Kolladiba 和 Debark 医院污水中分离出的细菌概况和抗菌药敏感性模式

本研究旨在调查从 Kolladiba 和 Debark 医院污水中分离出的一些多重耐药细菌的抗生素敏感性模式和细菌特征。从埃塞俄比亚北贡达尔的 Kolladiba 和 Debark 医院收集了 16 份样本，以调查是否存在多重耐药细菌。为了评估细菌的药敏模式，对分离良好的细菌菌落使用了七种抗生素。利用形态学和生化测试对筛选出的耐药分离菌进行了鉴定。此外，还对分离物进行了质粒 DNA 分析。在总共 28 个细菌分离物中，发现 12 个具有多重耐药性。在测试的抗生素中，红霉素是耐药性最强的抗生素，而新诺明则是最有效的抗生素。对分离菌株进行的质粒图谱研究显示，其中既存在质粒，也不存在质粒。质粒数量从 0 到 4 不等，质粒大小分别为 100、900、1,000、1,400、1,500 和 1,800 个碱基对。这项研究得出结论，两家医院的污水中都有大量耐多药的分离株。在这项研究中，细菌分离物中产生多重耐药性的基因可能是质粒介导的，也可能是染色体 DNA 介导的。这些污水中存在的耐多药细菌不容忽视。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.