The Preparation of Fluorescence Dual-Modality Nanosensor for Discrimination and Determination of Biothiols in Real Sample and Its Practical Detection Kit

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
İpek Ömeroğlu, VİLDAN ŞANKO, Ahmet Şenocak, Süreyya Oğuz Tümay
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Abstract

Biothiols widely exist in living organisms and have a crucial function in the human body for maintaining redox balance. It is vital yet difficult to develop probes that can detect and distinguish biothiols at the same time. This study presented the use of a very sensitive dual-modality nanosensor, NBD-Nap@NCC, for the discrimination and determination of biothiols in the real sample and its practical application based on RGB analysis by smartphone. The sensitive nanosensor was successfully prepared by surface modification of nanocrystalline cellulose (NCC), combining NBD and naphthalene fluorophores. Due to the high electron-withdrawing behavior of the NBD group which led to the PET mechanism between fluorophores, the prepared NBD-Nap@NCC nanosensor has a very weak fluorescence response. After treatment with Hcy or Cys, NBD-Nap@NCC quickly provided remarkably and in different rates of fluorescence “turn-on” response in both the blue and green channels, which was attributable to naphthalene and NBD fluorophores as a result of inhibition of PET mechanism. However, after treatment with GSH, only a significant blue-channel emission that was attributed to naphthalene fluorophore was obtained, indicating the inhibition of the PET mechanism. Furthermore, the NCC platform improves sensitivity and selectivity by increasing surface area and the number of binding sites due to modification of the NBD group on the surface. The detection limit ranged between 0.910 to 1.150 mol.L-1 for biothiols with large dynamic response ranges. The quantity of the Hcy, Cys and GSH were successfully determined in real sample after the evaluation of accuracy by HPLC and spike/recovery analysis. Additionally, paper-based analysis kits were fabricated for the practical detection of biothiols by RGB changes which were determined via the smartphone application.
用于鉴别和检测实际样品中生物硫醇的荧光双模态纳米传感器的制备及其实用检测试剂盒
生物硫醇广泛存在于生物体内,在人体中具有维持氧化还原平衡的重要功能。开发能够同时检测和区分生物硫醇的探针至关重要,但却很困难。本研究介绍了一种非常灵敏的双模态纳米传感器--NBD-Nap@NCC,用于分辨和测定真实样品中的生物硫醇,并基于智能手机的 RGB 分析进行了实际应用。通过对纳米结晶纤维素(NCC)进行表面修饰,结合 NBD 和萘荧光团,成功制备了这种灵敏的纳米传感器。由于 NBD 基团的高电子吸收行为导致荧光团之间的 PET 机制,制备的 NBD-Nap@NCC 纳米传感器的荧光响应非常微弱。经 Hcy 或 Cys 处理后,NBD-Nap@NCC 很快在蓝色和绿色通道中提供了不同速率的荧光 "开启 "响应,这是萘基和 NBD 荧光团抑制 PET 机制的结果。然而,经 GSH 处理后,只有萘荧光团发出明显的蓝色通道荧光,表明 PET 机制受到抑制。此外,由于表面 NBD 基团的修饰,NCC 平台增加了表面积和结合位点的数量,从而提高了灵敏度和选择性。生物硫醇的检测限在 0.910 至 1.150 mol.L-1 之间,动态响应范围较大。通过高效液相色谱法和加标/回收分析评估准确度后,成功测定了实际样品中 Hcy、Cys 和 GSH 的含量。此外,还制作了纸质分析工具包,用于通过 RGB 变化检测生物硫醇,并通过智能手机应用程序进行测定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
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